A Miniature Graphene-based Biosensor for Intracellular Glucose Measurements. (20th August 2015)

• Record Type: Journal Article
• Authors: Hasan, Kamran ul; Asif, Muhammad H.; Hassan, Muhammad Umair; Sandberg, Mats O.; Nur, O.; Willander, M.; Fagerholm, Siri; Strålfors, Peter
• Is Part Of: Electrochimica acta. Volume 174(2015)
• Page Start: 574
• Page End: 580
• Publication Date: 2015-08-20
• Subjects: Graphene -- Bio-sensors -- Glucose-oxidase -- Intracellular sensors -- Graphene oxide -- Glucose Sensor; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2015.06.035
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: We report on a small and simple graphene-based potentiometric sensor for the measurement of intracellular glucose concentration. A fine borosilicate glass capillary coated with graphene and subsequently immobilized with glucose oxidase (GOD) enzyme is inserted into the intracellular environment of a single human cell. The functional groups on the edge plane of graphene assist the attachment with the free amine terminals of GOD enzyme, resulting in a better immobilization. The sensor exhibits a glucose-dependent electrochemical potential against an Ag/AgCl reference microelectrode which is linear across the whole concentration range of interest (10 – 1000 μM). Glucose concentration in human fat cell measured by our graphene-based sensor is in good agreement with nuclear magnetic resonance (NMR) spectroscopy.