Manganese-superoxide dismutase (MnSOD), a role player in seahorse (Hippocampus abdominalis) antioxidant defense system and adaptive immune system. Issue 68 (September 2017)

• Record Type: Journal Article
• Authors: Perera, N.C.N.; Godahewa, G.I.; Lee, Seongdo; Kim, Myoung-Jin; Hwang, Jee Youn; Kwon, Mun Gyeong; Hwang, Seong Don; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 68(2017)
• Page Start: 435
• Page End: 442
• Publication Date: 2017-09
• Subjects: Antioxidant -- Immune response -- MnSOD -- XOD assay; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.07.049
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Manganese superoxide dismutase (MnSOD) is a metaloenzyme that catalyzes dismutation of the hazardous superoxide radicals into less hazardous H2 O2 and H2 O. Here, we identified a homolog of MnSOD from big belly seahorse ( Hippocampus abdominalis ; HaMnSOD ) and characterized its structural and functional features. HaMnSOD transcript possessed an open reading frame (ORF) of 672 bp which codes for a peptide of 223 amino acids. Pairwise alignment showed that HaMnSOD shared highest identity with rock bream MnSOD. Results of the phylogenetic analysis of HaMnSOD revealed a close proximity with rock bream MnSOD which was consistent with the result of homology alignment. The intense expression of HaMnSOD was observed in the ovary, followed by the heart and the brain. Further, immune related responses of HaMnSOD towards pathogenic stimulation were observed through bacterial and viral challenges. Highest HaMnSOD expression in response to stimulants Edwardsiella tarda, Streptococcus iniae, lipopolysaccharide (LPS), and polyinosinic-polycytidylic acid (Poly I:C) was observed in the late stage in the blood tissue. Xanthine/xanthine oxidase assay (XOD assay) indicated the ROS-scavenging ability of purified recombinant HaMnSOD (rHaMnSOD). The optimum conditions for the SOD activity of rHaMnSOD were pH 9 and the 25 °C. Collectively, the results obtained through the expressional analysis profiles and the functional assays provide insights into potential immune related and antioxidant roles of HaMnSOD in the big belly seahorse. Highlights: MnSOD was identified from big belly seahorse (HaMnSOD). HaMnSOD was cloned and expressed to evaluate its distinct functional features. XOD (Xanthine oxidase) assay confirmed the superoxide scavenging ability of HaMnSOD. Transcriptional level of HaMnSOD was modulated by pathological stress.