Mediation of coffee-induced improvements in human vascular function by chlorogenic acids and its metabolites: Two randomized, controlled, crossover intervention trials. Issue 6 (December 2017)

• Record Type: Journal Article
• Authors: Mills, Charlotte E.; Flury, Andreas; Marmet, Cynthia; Poquet, Laura; Rimoldi, Stefano F.; Sartori, Claudio; Rexhaj, Emrush; Brenner, Roman; Allemann, Yves; Zimmermann, Diane; Gibson, Glenn R.; Mottram, Don S.; Oruna-Concha, Maria-Jose; Actis-Goretta, Lucas; Spencer, Jeremy P.E.
• Is Part Of: Clinical nutrition. Volume 36:Issue 6(2017:Dec.)
• Page Start: 1520
• Page End: 1529
• Publication Date: 2017-12
• Subjects: Coffee -- Vascular -- Phenolics -- Flow mediated dilatation (FMD); 3-CQA 3-Caffeoylquinic acid -- 3-FQA 3 feruloylquinic acid -- 4-CQA 4-caffeoylquinic acid -- 4-CQ15L 4-caffeoylquinic-1, 5-lactone -- 4-FQA 4-feruloylquinic acid -- 4-MeCinA 4-methoxycinnamic acid -- 5-CQA 5-caffeoylquinic acid -- 5-FQA 5-feruloylquinic acid -- Ach acetylcholine -- ANOVA analysis of variance -- C3S caffeic-3′-O-sulfate -- CA4S caffeic-4′-O-sulfate -- CGA chlorogenic acid -- CV coefficient of variance -- CE collision cell entrance potential -- DP declustering potential -- ESI electrospray ionization -- EDTA ethylene-diamine-tetra-acetic acid -- FA ferulic acid -- F4G ferulic-4′-O-glucuronide -- F4S ferulic-4′-O-sulfate -- FMD flow mediated dilatation -- HDL high density lipoprotein -- HSD highest significant difference -- HPC high polyphenol coffee -- IAUC incremental area under the curve -- iFA isoferulic acid -- iF3G isoferulic-3′-O-glucuronide -- iF4S isoferulic-3′-O-sulfate -- LDI laser Doppler imaging -- LC liquid chromatography -- LDL low density lipoprotein -- LPC low polyphenol coffee -- MS mass spectrometry -- mCo3S m-coumaric acid-3′-O-sulfate -- mCo3G m-coumaric-3′-O-glucuronide -- MeFA methylferulic acid -- NIH National Institute of Health -- NO nitric oxide -- PDBP peripheral diastolic blood pressure -- PSBP peripheral systolic blood pressure -- POC proof of concept -- SNP sodium nitroprusside -- SD standard deviation -- SEM standard error of the mean; Critically ill -- Nutrition -- Periodicals; Diet therapy -- Periodicals; Parenteral feeding -- Periodicals; Enteral feeding -- Periodicals; Enteral Nutrition -- Periodicals; Parenteral Nutrition -- Periodicals; Metabolism -- Periodicals; Diétothérapie -- Périodiques; Alimentation parentérale -- Périodiques; Alimentation entérale -- Périodiques; Nutrition -- Périodiques; Diet therapy; Enteral feeding; Nutrition; Parenteral feeding; Electronic journals; Periodicals; Electronic journals; 615.854
• DOI: 10.1016/j.clnu.2016.11.013
• Languages: English
• ISSNs: 0261-5614

Abstract

Summary: Background & aims: Polyphenol intake has been linked to improvements in human vascular function, although data on hydroxycinnamates, such as chlorogenic acid (CGA) have not yet been studied. We aimed to investigate the impact of coffee intake rich in chlorogenic acid on human vascular function and whether CGAs are involved in potential effects. Methods: Two acute randomized, controlled, cross-over human intervention trials were conducted. The impact of coffee intake, matched for caffeine but differing in CGA content (89, and 310 mg) on flow-mediated dilatation (FMD) was assessed in 15 healthy male subjects. In a second intervention trial conducted with 24 healthy male subjects, the impact of pure 5-caffeoylquinic acid (5-CQA), the main CGA in coffee (5-CQA; 450 mg and 900 mg) on FMD was also investigated. Results: We observed a bi-phasic FMD response after low and high polyphenol, (89 mg and 310 mg CGA) intake, with increases at 1 (1.10 ± 0.43% and 1.34 ± 0.62%, respectively) and 5 (0.79% ± 0.32 and 1.52% ± 0.40, respectively) hours post coffee consumption. FMD responses to coffee intake was closely paralleled by the appearance of CGA metabolites in plasma, notably 3-, 4- and 5-feruloylquinic acid and ferulic-4′-O-sulfate at 1 h and isoferulic-3′-O-glucuronide and ferulic-4′-O-sulfate at 5 h. Intervention with purified 5-CQA (450 mg) also led to an improvement in FMD response relative to control (0.75 ± 1.31% at 1 h post intervention, p = 0.06) and concomitant appearance of plasma metabolites. Conclusions: Coffee intake acutely improves human vascular function, an effect, in part, mediated by 5-CQA and its physiological metabolites. Study registration: The National Institutes of Health (NIH) on ClinicalTrials.gov NCT01813981 and NCT01772784 . Highlights: Coffee is a rich source of phenolic acids, called chlorogenic acids, a subclass of (poly)phenols. Coffee induces biphasic improvements in endothelial function. Change in endothelial function correlates with plasma chlorogenic acid metabolites.