A single WAP domain-containing protein from Litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against AHPND-inducing Vibrio parahaemolyticus. Issue 68 (September 2017)

• Record Type: Journal Article
• Authors: Visetnan, Suwattana; Supungul, Premruethai; Tassanakajon, Anchalee; Donpudsa, Suchao; Rimphanitchayakit, Vichien
• Is Part Of: Fish & shellfish immunology. Issue 68(2017)
• Page Start: 341
• Page End: 348
• Publication Date: 2017-09
• Subjects: LvSWD -- Crustin -- Antimicrobial protein -- AHPND-inducing Vibrio parahaemolyticus; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.07.046
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The single WAP domain-containing protein (SWD) is a type III crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. A study of SWD from Litopenaeus vannamei ( Lv SWD) is reported herein about its activities and function against bacteria, particularly the AHPND-inducing Vibrio parahaemolyticus ( VP AHPND ) that causes acute hepatopancreatic necrosis disease (AHPND). The Lv SWD is mainly synthesized in hemocytes and up-regulated in response to VP AHPND infection. Over-expressed mature recombinant Lv SWD (r Lv SWD) and its WAP domain (r Lv SWD-WAP) are able to strongly inhibit subtilisin but not trypsin, chymotrypsin and elastase. The r Lv SWD inhibits subtilisin with the inhibition constant ( K i ) of 14.3 nM. However, only r Lv SWD exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. Unlike the r Lv SWD, the r Lv SWD-WAP does not possess antimicrobial activity. Therefore, the killing effect of r Lv SWD on VP AHPND and Bacillus megaterium was studied. The MIC of 30 μM against VP AHPND is bactericidal whereas the MIC against B. megaterium is not. With four times the MIC of r Lv SWD, the VP AHPND -treated post larval shrimp are able to survive longer with 50% survival rate as long as 78 h as compared to 36 h of the infected shrimp without r Lv SWD. The antimicrobial activity of Lv SWD against the VP AHPND infection suggests its potential application for disease control in aquaculture. Highlights: The LvSWD is up-regulated in response to VP AHPND infection. The r Lv SWD and its WAP domain are able to strongly inhibit subtilisin. The r Lv SWD but not the WAP domain exhibits antimicrobial activity against both Gram-positive and Gram-negative bacteria. The r Lv SWD-treated post larval shrimp infected by VP AHPND are able to survive longer.