Dioscin alleviates non-alcoholic fatty liver disease through adjusting lipid metabolism via SIRT1/AMPK signaling pathway. (May 2018)

• Record Type: Journal Article
• Authors: Yao, Hong; Tao, Xufeng; Xu, Lina; Qi, Yan; Yin, Lianhong; Han, Xu; Xu, Youwei; Zheng, Lingli; Peng, Jinyong
• Is Part Of: Pharmacological research. Volume 131(2018)
• Page Start: 51
• Page End: 60
• Publication Date: 2018-05
• Subjects: ALT alanine aminotransferase -- AMPK adenosine 5′-monophosphate activated protein kinase -- AST aspartate aminotransferase -- ATGL adipose triglyceride lipase -- BCA bicinchoninic acid -- CMC-Na carboxymethylcellulose sodium -- CPT carnitine palmitoyltransferase -- DAPI 4′, 6′-diamidino-2-phenylindole -- DMSO dimethylsulfoxide -- ECL enhanced chemiluminescence -- ELISA enzyme-linked immunosorbent assay -- FAS fatty acid synthase -- FBG fasting blood glucose -- FBS fetal bovine serum -- FFA liver tissue free fatty acid -- FoxO1 forkhead box O 1 -- GSH glutathione -- H&E hematoxylin and eosin -- MDA malondialdehyde -- NAD+ nicotinamide adenine dinucleotide -- NAFLD non-alcoholic fatty liver disease -- NASH non-alcoholic steatohepatitis -- OGTT oral glucose tolerance test -- PA palmic acid -- SCD stearoyl-CoA desaturase -- SD standard deviation -- SIRT1 silent information regulator of transcription 1 -- SOD superoxide dismutase -- SREBP-1c sterol-regulatory element binding protein-1c -- TC total cholesterol -- TEM transmission electron microscopy -- TG total triglyceride; AMPK -- Dioscin -- Lipid metabolism -- NAFLD -- SIRT1; Pharmacology -- Periodicals; Pharmacology -- Periodicals; Research -- Periodicals; Médicaments -- Recherche -- Périodiques; Pharmacologie -- Périodiques; 615.105
• DOI: 10.1016/j.phrs.2018.03.017
• Languages: English
• ISSNs: 1043-6618

Abstract

Graphical abstract: Abstract: Dioscin, one natural product, has active effect against non-alcoholic fatty liver disease (NAFLD) in our previous work. However, the pharmacological data are insufficient and the mechanisms have not been reported. Thus, this study aims to comprehensively investigate the effects and molecular mechanisms of dioscin against NAFLD. The primary cultured hepatocytes, AML-12 and HepG-2 cells were treated with palmic acid (PA) after dioscin treatment. The mice and rats were induced by high fat diet to establish the in vivo models of NAFLD. Dioscin obviously alleviated liver lipid accumulation symptoms and improved the levels of serum and hepatic biochemical parameters in vitro and in vivo . Further investigations revealed that dioscin significantly attenuated lipid metabolism via adjusting SIRT1/AMPK signal pathway to regulate the expression levels of SREBP-1c, CPT, FAS, SCD, FoxO1 and ATGL. In addition, suppression of SIRT1 by Nicotinamide or abrogation of AMPK by Compound C eliminated the inhibitory effects of dioscin on lipid metabolism. Therefore, our findings further demonstrated that dioscin markedly prevented NAFLD through adjusting lipid metabolism via SIRT1/AMPK signal pathway, which should be developed as a new candidate for NAFLD.