Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp. (September 2018)

• Record Type: Journal Article
• Authors: Zhang, Yi; Kong, Xiaoying; Wang, Zhongming; Sun, Yongming; Zhu, Shunni; Li, Lianhua; Lv, Pengmei
• Is Part Of: Renewable energy. Volume 125(2018)
• Page Start: 1049
• Page End: 1057
• Publication Date: 2018-09
• Subjects: Cell wall disruption -- Enzymatic hydrolysis -- Lipid extraction yield -- Microalgae Scenedesmus sp. -- Optimization -- Biodiesel; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2018.01.078
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: Cell wall disruption is an essential downstream processing step for improving the efficiency of lipid extraction from microalgae. Enzyme-assisted extraction of lipid from microalga Scenedesmus sp. with cellulase, xylanase and pectinase, using various parameters, such as enzyme concentration, temperature, pH and incubation time, was optimized by central composite design (CCD) coupled with response surface methodology (RSM). Both the lipid extraction from microalgae and the fatty acid methyl esters (FAMEs) production under optimal conditions showed a ∼twofold in the yields compared to the control group with no enzymatic treatment. SEM images, FTIR measurement, XPS and HPLC analysis showed that the enzymatic pretreatment caused significant alterations in the cell wall structure of microalgae. And the disruption of microalgal cell walls was primarily attributed to the breakage of β-glucosidic linkages in cellulose and hemicellulose. The study showed a promising approach can lead to an improvement in the lipid extraction yield from microalgae and further provide valuable information for the use of enzymes in microalgal processes. Highlights: Enzymatic hydrolysis for microalgae cell disruption was studied. Six process parameters were optimized by respond surface method. Enzymatic hydrolysis can effectively improve the microalgae biodiesel yield. Microalgae cell walls were significantly destroyed by multi-enzymes treatment. The characteristic of cell walls were analyzed by SEM, FTIR, XPS and HPLC.