High‐level production of d‐pantothenic acid from glucose by fed‐batch cultivation of Escherichia coli. (11th October 2020)

• Record Type: Journal Article
• Authors: Zou, Shu‐Ping; Wang, Zhi‐Jian; Zhao, Kuo; Zhang, Bo; Niu, Kun; Liu, Zhi‐Qiang; Zheng, Yu‐Guo
• Is Part Of: Biotechnology and applied biochemistry. Volume 68:Number 6(2021)
• Page Start: 1227
• Page End: 1235
• Publication Date: 2020-10-11
• Subjects: d‐pantothenic acid -- fed‐batch culture -- glucose control -- isoleucine -- optimal feeding strategy; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2044
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: d ‐Pantothenic acid (D‐PA) is an essential vitamin widely used in food, feed, chemical, and pharmaceutical industries. An Escherichia coli platform was developed for the high‐level production of D‐PA from glucose through fed‐batch cultivation. Initially, the effects of different glucose feeding strategies D‐PA synthesis were studied. It was found that D‐PA production in glucose control (5 g/L) fed‐batch culture reached 24.3 g/L, which was 4.09 times that in the batch culture. Next, the effect of auxotrophic amino acid (isoleucine)‐limited feeding on D‐PA production was investigated. The results revealed that isoleucine feeding decreased the accumulation of by‐product acetic acid and promoted D‐PA production significantly. Furthermore, an isoleucine feeding embedded multistage glucose supply strategy was established, and a maximum titer of 39.1 g/L was achieved. To the best of our knowledge, this levels are the highest reported so far in engineered E. coli for the D‐PA production. The developed fed‐batch feeding strategy may be useful for the industrial D‐PA production by E. coli . Abstract : Production of D‐PA from glucose in metabolically engineered Escherichia coli strains through using isoleucine feeding embedded multistage glucose supply strategy