Unique features of the m6A methylome and its response to drought stress in sea buckthorn (Hippophae rhamnoides Linn.). (12th November 2021)

• Record Type: Journal Article
• Authors: Zhang, Guoyun; Lv, Zhongrui; Diao, Songfeng; Liu, Hong; Duan, Aiguo; He, Caiyun; Zhang, Jianguo
• Is Part Of: RNA biology. Volume 18(2021)Supplement 2
• Page Start: 794
• Page End: 803
• Publication Date: 2021-11-12
• Subjects: Sea buckthorn -- N6-methyladenosine -- drought stress -- m6A modification -- m6A demethylase; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2021.1992996
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: In plants, recent studies have revealed that N6-methyladenosine (m 6 A) methylation of mRNA has potential regulatory functions of this mRNA modification in many biological processes. m 6 A methyltransferase, m 6 A demethylase and m 6 A-binding proteins can cause differential phenotypes, indicating that m 6 A may have critical roles in the plant. In this study, we depicted the m 6 A map of sea buckthorn ( Hippophae rhamnoides Linn.) transcriptome. Similar to A. thaliana, m 6 A sites of sea buckthorn transcriptome is significantly enriched around the stop codon and within 3ʹ-untranslated regions (3ʹUTR). Gene ontology analysis shows that the m 6 A modification genes are associated with metabolic biosynthesis. In addition, we identified 13, 287 different m 6 A peaks (DMPs) between leaf under drought (TR) and control (CK) treatment. It reveals that m 6 A has a high level of conservation and has a positive correlation with mRNA abundance in plants. GO and KEGG enrichment results showed that DMP modification DEGs in TR were particularly associated with ABA biosynthesis. Interestingly, our results showed three m 6 A demethylase ( HrALKBH10B, HrALKBH10C and HrALKBH10D ) genes were significantly increased following drought stress, which indicated that it may contributed the decreased m 6 A levels. This exhaustive m 6 A map provides a basis and resource for the further functional study of mRNA m 6 A modification in abiotic stress.