Genome Annotation and Curation Using MAKER and MAKER‐P. Issue 1 (12th December 2014)

• Record Type: Journal Article
• Authors: Campbell, Michael S.; Holt, Carson; Moore, Barry; Yandell, Mark
• Editors: Bateman, Alex; Pearson, William R.; Stein, Lincoln D.; Stormo, Gary D.; Yates, John R.
• Is Part Of: Current protocols in bioinformatics. Volume 48:Issue 1(2014)
• Page Start: 4.11.1
• Page End: 4.11.39
• Publication Date: 2014-12-12
• Subjects: genome annotation -- comparative genomics -- gene finding -- plants; Bioinformatics -- Laboratory manuals; Nucleotide sequence -- Laboratory manuals; Amino acid sequence -- Laboratory manuals; Base Sequence; Amino Acid Sequence; Computational Biology -- methods; Databases, Genetic; Proteins -- analysis; Sequence Analysis -- methods; Sequence Homology; Amino acid sequence; Bioinformatics; Nucleotide sequence; Laboratory Manuals; Laboratory manuals; 570.285
• DOI: 10.1002/0471250953.bi0411s48
• Languages: English
• ISSNs: 1934-3396

Abstract

Abstract: This unit describes how to use the genome annotation and curation tools MAKER and MAKER‐P to annotate protein‐coding and noncoding RNA genes in newly assembled genomes, update/combine legacy annotations in light of new evidence, add quality metrics to annotations from other pipelines, and map existing annotations to a new assembly. MAKER and MAKER‐P can rapidly annotate genomes of any size, and scale to match available computational resources. © 2014 by John Wiley & Sons, Inc.