Alkaline Ceramidase Mediates the Oxidative Stress Response in Drosophila melanogaster Through Sphingosine. Issue 3 (22nd May 2019)

• Record Type: Journal Article
• Authors: Zhang, Chun-Hong; Zhang, Min-Jing; Shi, Xiao-Xiao; Mao, Cungui; Zhu, Zeng-Rong
• Editors: Swale, Daniel
• Is Part Of: Journal of insect science. Volume 19:Issue 3(2019)
• Publication Date: 2019-05-22
• Subjects: ceramidase -- Dacer -- antioxidative -- catalase -- sphingosine; Insects -- Periodicals; Entomology -- Periodicals; Arthropoda -- Periodicals; Insects; Arthropods; Biology; Ecology; Entomologie -- Périodiques; Entomology; Insects; Electronic journals; Periodicals; 595.05
• DOI: 10.1093/jisesa/iez042
• Languages: English
• ISSNs: 1536-2442

Abstract

Abstract: Alkaline ceramidase ( Dacer ) in Drosophila melanogaster was demonstrated to be resistant to paraquat-induced oxidative stress. However, the underlying mechanism for this resistance remained unclear. Here, we showed that sphingosine feeding triggered the accumulation of hydrogen peroxide (H2 O2 ). Dacer -deficient D. melanogaster ( Dacer mutant) has higher catalase (CAT) activity and CAT transcription level, leading to higher resistance to oxidative stress induced by paraquat. By performing a quantitative proteomic analysis, we identified 79 differentially expressed proteins in comparing Dacer mutant to wild type. Three oxidoreductases, including two cytochrome P450 ( CG3050, CG9438 ) and an oxoglutarate/iron-dependent dioxygenase ( CG17807 ), were most significantly upregulated in Dacer mutant. We presumed that altered antioxidative activity in Dacer mutant might be responsible for increased oxidative stress resistance. Our work provides a novel insight into the oxidative antistress response in D. melanogaster .