Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli. Issue 3 (10th January 2022)

• Record Type: Journal Article
• Authors: Mimura, Soma; Shimizu, Tomohiro; Shingubara, Shoso; Iwaki, Hiroaki; Ito, Takeshi
• Is Part Of: RSC advances. Volume 12:Issue 3(2022)
• Page Start: 1645
• Page End: 1652
• Publication Date: 2022-01-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra07623j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The time profiles of active cell ratios depended on the growth phase and the absence of some lytic transglycosylases of E. coli . Significant cell damage was not found on the autolysis inhibition condition. Abstract : Nanostructures exhibit a bactericidal effect owing to physical interaction with the bacterial cell envelope. Here, we aimed to identify the mechanism underlying the bactericidal effect of nanostructures based on bacterial autolysis, in contrast to previous reports focusing on structural characteristics. The time profiles of active cell ratios of the Escherichia coli strains (WT, Δ mltA, Δ mltB, Δ slt 70), incubation time of the wild-type (WT) strains, and autolysis inhibition of WT strains were evaluated with respect to the bactericidal effect of the applied nanostructures. Addition of Mg 2+, an autolysis inhibitor, was not found to cause significant cell damage. The incubation phase was significantly associated with envelope damage. The lytic transglycosylase-lacking strain of Slt70 (Δ slt 70) also showed only minimal envelope damage. Our results indicate that nanostructures may act by triggering bacterial autolysis.