Deactivation of a dimeric DNA-binding peptide through a palladium-mediated self-immolative cleavage. Issue 6 (26th January 2022)

• Record Type: Journal Article
• Authors: Rodríguez, Jessica; Pérez-González, Cibrán; Martínez-Calvo, Miguel; Mosquera, Jesús; Mascareñas, José L.
• Is Part Of: RSC advances. Volume 12:Issue 6(2022)
• Page Start: 3500
• Page End: 3504
• Publication Date: 2022-01-26
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra09180h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A metallic trigger for disassembling and cancelling function. Abstract : Herein, we describe an approach for the on-demand disassembly of dimeric peptides using a palladium-mediated cleavage of a designed self-immolative linker. The utility of the strategy is demonstrated for the case of dimeric basic regions of b ZIP transcription factors. While the dimer binds designed DNA sequences with good affinities, the peptide–DNA complex can be readily dismounted by addition of palladium reagents that trigger the cleavage of the spacer, and the release of unfunctional monomeric peptides.