In Vitro T-Cell Generation From Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination. (11th August 2015)

• Record Type: Journal Article
• Authors: Smith, Michelle J.; Webber, Beau R.; Mohtashami, Mahmood; Stefanski, Heather E.; Zún˜iga-Pflücker, Juan Carlos; Blazar, Bruce R.
• Is Part Of: Stem cells. Volume 33:Number 11(2015:Nov.)
• Page Start: 3174
• Page End: 3180
• Publication Date: 2015-08-11
• Subjects: Induced pluripotent stem cells -- CD34 + -- Cell culture -- Cord blood -- Differentiation -- Embryonic stem cells -- Hematopoietic stem cells -- T cells; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2115
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: T lymphocytes are critical mediators of the adaptive immune system and have the capacity to serve as therapeutic agents in the areas of transplant and cancer immunotherapy. While T cells can be isolated and expanded from patients, T cells derived in vitro from both hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (hPSCs) offer great potential advantages in generating a self-renewing source of T cells that can be readily genetically modified. T-cell differentiation in vivo is a complex process requiring tightly regulated signals; providing the correct signals in vitro to induce T-cell lineage commitment followed by their development into mature, functional, single positive T cells, is similarly complex. In this review, we discuss current methods for the in vitro derivation of T cells from murine and human HSPCs and hPSCs that use feeder-cell and feeder-cell-free systems. Furthermore, we explore their potential for adoption for use in T-cell-based therapies. Stem Cells 2015;33:3174–3180