Delayed Minimally Invasive Injection of Allogenic Bone Marrow Stromal Cell Sheets Regenerates Large Bone Defects in an Ovine Preclinical Animal Model. (1st April 2015)

• Record Type: Journal Article
• Authors: Berner, Arne; Henkel, Jan; Woodruff, Maria A.; Steck, Roland; Nerlich, Michael; Schuetz, Michael A.; Hutmacher, Dietmar W.
• Is Part Of: Stem cells translational medicine. Volume 4:Number 5(2015)
• Page Start: 503
• Page End: 512
• Publication Date: 2015-04-01
• Subjects: Large bone defect -- Mesenchymal stem cells -- Allogenic -- Bone tissue engineering -- Cell injection -- Bone regeneration -- Sheep; Stem cells -- Periodicals; Regenerative medicine -- Periodicals; Periodicals; 616.0277405
• DOI: 10.5966/sctm.2014-0244
• Languages: English
• ISSNs: 2157-6564

Abstract

Abstract: : Cell-based tissue engineering approaches are promising strategies in the field of regenerative medicine. However, the mode of cell delivery is still a concern and needs to be significantly improved. Scaffolds and/or matrices loaded with cells are often transplanted into a bone defect immediately after the defect has been created. At this point, the nutrient and oxygen supply is low and the inflammatory cascade is incited, thus creating a highly unfavorable microenvironment for transplanted cells to survive and participate in the regeneration process. We therefore developed a unique treatment concept using the delayed injection of allogenic bone marrow stromal cell (BMSC) sheets to regenerate a critical-sized tibial defect in sheep to study the effect of the cells' regeneration potential when introduced at a postinflammatory stage. Minimally invasive percutaneous injection of allogenic BMSCs into biodegradable composite scaffolds 4 weeks after the defect surgery led to significantly improved bone regeneration compared with preseeded scaffold/cell constructs and scaffold-only groups. Biomechanical testing and microcomputed tomography showed comparable results to the clinical reference standard (i.e., an autologous bone graft). To our knowledge, we are the first to show in a validated preclinical large animal model that delayed allogenic cell transplantation can provide applicable clinical treatment alternatives for challenging bone defects in the future. Significance: From a translational point of view, a comprehensive study is presented, the results of which show that percutaneous injection of allogenic BMSCs into the biodegradable composite scaffold 4 weeks after the defect surgery led to significantly improved bone regeneration compared with preseeded scaffold/cell constructs and scaffold-only groups. Biomechanical testing and microcomputed tomography showed results comparable to those of the clinical gold standard, namely autologous autograft. To the authors' knowledge, this is the first study to display in a validated preclinical large animal model that delayed allogenic cell transplantation could provide clinical treatment alternatives for challenging bone defects in the future. Abstract : A unique treatment concept was developed using delayed injection of allogenic bone marrow stromal cell (BMSC) sheets to regenerate critical-sized tibial defects in sheep to analyze their regeneration potential. Delayed injection of allogenic BMSCs into biodegradable composite scaffolds 4 weeks after defect surgery led to significantly improved bone regeneration compared with direct transplantation of scaffold/cell constructs into the defect.