Interrogation of a Context-Specific Transcription Factor Network Identifies Novel Regulators of Pluripotency. (22nd January 2015)

• Record Type: Journal Article
• Authors: Kushwaha, Ritu; Jagadish, Nirmala; Kustagi, Manjunath; Tomishima, Mark J.; Mendiratta, Geetu; Bansal, Mukesh; Kim, Hyunjae R.; Sumazin, Pavel; Alvarez, Mariano J.; Lefebvre, Celine; Villagrasa-Gonzalez, Patricia; Viale, Agnes; Korkola, James E.; Houldsworth, Jane; Feldman, Darren R.; Bosl, George J.; Califano, Andrea; Chaganti, R. S. K.
• Is Part Of: Stem cells. Volume 33:Number 2(2015:Feb.)
• Page Start: 367
• Page End: 377
• Publication Date: 2015-01-22
• Subjects: Biomathematical modeling -- Differentiation -- Embryonal carcinoma -- Embryonic stem cells -- Gene expression -- Pluripotent stem cells; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.1870
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: The predominant view of pluripotency regulation proposes a stable ground state with coordinated expression of key transcription factors (TFs) that prohibit differentiation. Another perspective suggests a more complexly regulated state involving competition between multiple lineage-specifying TFs that define pluripotency. These contrasting views were developed from extensive analyses of TFs in pluripotent cells in vitro. An experimentally validated, genome-wide repertoire of the regulatory interactions that control pluripotency within the in vivo cellular contexts is yet to be developed. To address this limitation, we assembled a TF interactome of adult human male germ cell tumors (GCTs) using the Algorithm for the Accurate Reconstruction of Cellular Pathways (ARACNe) to analyze gene expression profiles of 141 tumors comprising pluripotent and differentiated subsets. The network (GCT Net ) comprised 1, 305 TFs, and its ingenuity pathway analysis identified pluripotency and embryonal development as the top functional pathways. We experimentally validated GCT Net by functional (silencing) and biochemical (ChIP-seq) analysis of the core pluripotency regulatory TFs POU5F1, NANOG, and SOX2 in relation to their targets predicted by ARACNe. To define the extent of the in vivo pluripotency network in this system, we ranked all TFs in the GCT Net according to sharing of ARACNe-predicted targets with those of POU5F1 and NANOG using an odds-ratio analysis method. To validate this network, we silenced the top 10 TFs in the network in H9 embryonic stem cells. Silencing of each led to downregulation of pluripotency and induction of lineage; 7 of the 10 TFs were identified as pluripotency regulators for the first time. Stem Cells 2015;33:367–377