Anti-Ace monoclonal antibody reduces Enterococcus faecalis aortic valve infection in a rat infective endocarditis model. Issue 8 (16th November 2018)

• Record Type: Journal Article
• Authors: Singh, Kavindra V; Pinkston, Kenneth L; Gao, Peng; Harvey, Barrett R; Murray, Barbara E
• Is Part Of: Pathogens and disease. Volume 76:Issue 8(2018)
• Publication Date: 2018-11-16
• Subjects: Enterococcus faecalis -- pathogenesis -- ace collagen adhesion -- infective endocarditis -- protective vaccine -- immunoprophylaxis; Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1093/femspd/fty084
• Languages: English
• ISSNs: 2049-632X

Abstract

Abstract: Ace (Adhesin to collagen from Enterococcus faecalis ) is a cell-wall anchored protein that is expressed conditionally and is important for virulence in a rat infective endocarditis (IE) model. Previously, we showed that rats immunized with the collagen binding domain of Ace (domain A), or administered anti-Ace domain A polyclonal antibody, were less susceptible to E. faecalis endocarditis than sham-immunized controls. In this work, we demonstrated that a sub nanomolar monoclonal antibody (mAb), anti-Ace mAb70, significantly diminished E. faecalis binding to ECM collagen IV in in vitro adherence assays and that, in the endocarditis model, anti-Ace mAb70 pre-treatment significantly reduced E. faecalis infection of aortic valves. The effectiveness of anti-Ace mAb against IE in the rat model suggests it might serve as a beneficial agent for passive protection against E. faecalis infections. Abstract : Anti-Ace mAb70 prevents E. faecalis endocarditis in rats.