Pinisolibacter aquiterrae sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from benzene-, and xylene-degrading enrichment cultures, and emended description of the genus Pinisolibacter. Issue 2 (9th February 2022)

• Record Type: Journal Article
• Authors: Bedics, Anna; Banerjee, Sinchan; Bóka, Károly; Tóth, Erika; Benedek, Tibor; Kriszt, Balázs; Táncsics, András
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 2(2022)
• Publication Date: 2022-02-09
• Subjects: Pinisolibacter aquiterrae -- new taxa -- Hyphomicrobiales -- BTEX degradation; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005229
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two Gram-reaction-negative strains, designated as B13 T and MA2-2, were isolated from two different aromatic hydrocarbon-degrading enrichment cultures and characterized using a polyphasic approach to determine their taxonomic position. The two strains had identical 16S rRNA gene sequences and were most closely related to Pinisolibacter ravus E9 T (97.36 %) and Siculibacillus lacustris SA-279 T (96.33 %). Cells were facultatively aerobic rods and motile with a single polar flagellum. The strains were able to degrade ethylbenzene as sole source of carbon and energy. The assembled genome of strain B13 T had a total length of 4.91 Mb and the DNA G+C content was 68.8 mol%. The predominant fatty acids (>5 % of the total) of strains B13 T and MA2-2 were C18 : 1 ω 7 c /C18 : 1 ω 6 c, C16 : 1 ω 7 c /C16 : 1 ω 6 c and C16 : 0 . The major ubiquinone of strain B13 T was Q10, while the major polar lipids were phosphatidyl- N -methylethanolamine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and a phospholipid. Based on phenotypic characteristics and phylogenetic data, it is concluded that strains B13 T and MA2-2 are members of the genus Pinisolibacter and represent a novel species for which the name Pinisolibacter aquiterrae sp. nov. is proposed. The type strain of the species is strain B13 T (=LMG 32346 T =NCAIM B.02665 T ).