Interleukin-36γ Is Elevated in Cervicovaginal Epithelial Cells in Women With Bacterial Vaginosis and In Vitro After Infection With Microbes Associated With Bacterial Vaginosis. (5th October 2019)

• Record Type: Journal Article
• Authors: Gardner, Jameson K; Łaniewski, Paweł; Knight, Anna; Haddad, Lisa B; Swaims-Kohlmeier, Alison; Herbst-Kralovetz, Melissa M
• Is Part Of: Journal of infectious diseases. Volume 221:Number 6(2020)
• Page Start: 983
• Page End: 988
• Publication Date: 2019-10-05
• Subjects: Atopobium vaginae -- Gardnerella vaginalis; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiz514
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: In recent studies, the interleukin (IL)-36 cytokines were shown to be elevated in women with non- Lactobacillus- dominated vaginal microbiomes. In this study, we evaluated IL36G expression in clinical samples from women with and without bacterial vaginosis (BV) and a human 3-dimensional cervical epithelial cell model. IL36G expression was significantly elevated in cervicovaginal epithelial cells isolated from BV-positive women and corresponded with increased neutrophil counts relative to BV-negative women. In addition, specific BV-associated bacterial species as well as a polymicrobial cocktail significantly induced IL36G expression in vitro. These findings suggest that IL-36γ may exhibit an important function in the host response to BV and other sexually transmitted infections. Abstract : IL36G expression is elevated in cervicovaginal epithelial cells from women with BV relative to women without BV. Moreover, IL36G is induced by individual BV-associated bacteria in a species-specific manner and a polymicrobial cocktail in vitro using a human 3D model.