Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. (13th July 2017)

• Record Type: Journal Article
• Authors: Franco-Garcia, Alexandra; Varughese, Tilly A; Lee, Yeon Joo; Papanicolaou, Genovefa; Rosenblum, Marc K; Hollmann, Travis J; Koehne, Guenther; Boulad, Farid; Babady, N Esther; Tang, Yi-Wei; Seo, Susan K
• Is Part Of: Open forum infectious diseases. Volume 4:Number 3(2017)
• Publication Date: 2017-07-13
• Subjects: 16S ribosomal ribonucleic acid gene sequencing -- allogeneic hematopoietic cell transplant -- extrapulmonary legionellosis -- matrix-assisted laser desorption/ionization time-of-flight mass spectrometry -- molecular diagnostics; Communicable diseases -- Periodicals; Medical microbiology -- Periodicals; Infection -- Periodicals; 616.9
• DOI: 10.1093/ofid/ofx140
• Languages: English
• ISSNs: 2328-8957

Abstract

Abstract: Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.