Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis. Issue 11 (24th November 2020)

• Record Type: Journal Article
• Authors: Schwitalla, Jan W.; Benndorf, René; Martin, Karin; Vollmers, John; Kaster, Anne-Kristin; de Beer, Z. Wilhelm; Poulsen, Michael; Beemelmanns, Christine
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 11(2020)
• Page Start: 5806
• Page End: 5811
• Publication Date: 2020-11-24
• Subjects: Macrotermes natalensis -- Streptomyces -- termite gut; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004478
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5 T, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite Macrotermes natalensis, showed morphological, physiological and chemotaxonomic properties typical of the genus Streptomyces . Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5 T was Streptomyces polyrhachis DSM 42102 T (98.87 %). DNA–DNA hybridization experiments between strain RB5 T and S. polyrhachis DSM 42102 T resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5 T contained ll -diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H6 ) and MK-9(H8 )]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C14 : 0 ) 12-methyltetradecanoic acid (anteiso-C15 : 0 ), 13-methyltetradecanoic acid (iso-C15 : 0 ) and 14-methylpentadecanoic acid (iso-C16 : 0 ). Here, we propose a novel species of the genus Streptomyces – Streptomyces smaragdinus with the type strain RB5 T (=VKM Ac-2839 T =NRRL B65539 T ).