Cerasibacillus terrae sp. nov., isolated from maize field, and emended description of Cerasibacillus quisquiliarum Nakamura et al. 2004. Issue 11 (17th November 2020)

• Record Type: Journal Article
• Authors: Lin, Shih-Yao; Tsai, Chia-Fang; Hameed, Asif; Young, Chiu-Chung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 11(2020)
• Page Start: 5725
• Page End: 5733
• Publication Date: 2020-11-17
• Subjects: average nucleotide identity (ANI) -- Cerasibacillus terrae -- digital DNA–DNA hybridization (dDDH) -- strain CC-CFT480T; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004470
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT480 T, isolated from soil sampled in a maize field in Taiwan, ROC. Cells of strain CC-CFT480 T were rod-shaped, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 °С, pH 8 and 3 % NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT480 T associated with Cerasibacillus quisquiliarum (97.2 % sequence identity), Virgibacillus soli (95.7 %), Virgibacillus carmonensis (95.4 %) and Virgibacillus byunsanensis (95.2 %), and lower sequence similarity values to other species. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain CC-CFT480 T and C. quisquiliarum were 74.2 and 20.1 %, respectively. Strain CC-CFT480 T contained iso-C15:0, C16:1 ω7 c alcohol and iso-C17:1 ω 10 c as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown aminophospholipids, one uncharacterized aminophospholipid and two unknown phospholipids. The major polyamine was spermidine. The DNA G+C content was 34.8 mol% and the predominant quinone was menaquinone 7 (MK-7). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, ANI and dDDH analyses, strain CC-CFT480 T is proposed to represent a novel Cerasibacillus species, for which the name Cerasibacillus terrae sp. nov. (type strain CC-CFT480 T =BCRC 81216 T =JCM 33498 T ).