Streptomyces arboris sp. nov., isolated from Populus euphratica wetland soil. Issue 11 (17th November 2020)

• Record Type: Journal Article
• Authors: Liu, Panpan; Xia, Zhanfeng; Zhang, Lili
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 11(2020)
• Page Start: 5613
• Page End: 5619
• Publication Date: 2020-11-17
• Subjects: chemotaxonomic -- gene -- Populus euphratica -- phylogenetic -- strain -- Streptomyces; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004430
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel actinobacterial strain (TRM 68085 T ) was isolated from soil of Populus euphratica wetland. A polyphasic approach was used to study the taxonomy of TRM 68085 T and the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085 T showed the highest similarity value to Streptomyces capitiformicae 1H-SSA4 T (98.6 %), and phylogenetically clustered with Streptomyces kanasensis ZX01 T (97.5 %) and Streptomyces ipomoeae NBRC 13050 T (97.4 %). The genomic DNA G+C content of strain TRM 68085 T based on the genome sequence was 71.4 mol%. The levels of DNA–DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours, S. capitiformicae 1H-SSA4 T, S. kanasensis ZX01 T and S. ipomoeae NBRC 13050 T, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085 T possessed MK-9(H6 ) and MK-9(H8 ) as the predominant menaquinones. ll -Diaminopimelic acid and a small amount of meso -diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C16 : 0 (25.4 %) and iso-C16 : 0 (18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085 T represents a novel species of the genus Streptomyces, for which the name Streptomyces arboris sp. nov. is proposed. The type strain is TRM 68085 T (=CCTCC AA2019031 T =LMG 31492 T ).