Pseudokineococcus galaxeicola sp. nov., isolated from mucus of a stony coral. Issue 11 (8th November 2020)

• Record Type: Journal Article
• Authors: Li, Qiqi; Zheng, Yicong; Guo, Anjie; Chen, Yu; Zhang, Si; Li, Jie
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 11(2020)
• Page Start: 5671
• Page End: 5675
• Publication Date: 2020-11-08
• Subjects: coral mucus -- polyphasic taxonomy -- Pseudokineococcus -- Pseudokineococcus galaxeicola sp. nov.; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004460
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, aerobic, motile, coccus-shaped bacterium, designated strain SCSIO 13315 T, was isolated from mucus of the coral Galaxea sp. collected from Luhuitou fringing reef (Sanya, Hainan, PR China). Analysis of the 16S rRNA gene sequence showed that strain SCSIO 13315 T exhibits 95.5 % 16S rRNA gene sequence similarity to Pseudokineococcus basanitobsidens SKC1-2 T, 95.8 % to Pseudokineococcus lusitanus T2A-S27 T and 96.4 % to Pseudokineococcus marinus KST3-3 T . Results of phylogenetic analysis suggested that strain SCSIO 13315 T was a member of the genus Pseudokineococcus . The DNA G+C content of strain SCSIO 13315 T was 73.5 %. Chemotaxonomic assessment of strain SCSIO 13315 T showed that the menaquinones were MK-8(H4 ) and MK-9(H2 ). The main cellular fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω 7 c /C16 : 1 ω 6 c ). The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids, four unidentified aminolipids and one unidentified lipid. Based on the phylogenetic and phenotypic analysis, it was evident that strain SCSIO 13315 T represents a novel species of the genus Pseudokineococcus, for which the name Pseudokineococcus galaxeicola sp. nov. is proposed. The type strain is SCSIO 13315 T (=NBRC 109944 T =DSM 27812 T ).