A Sensitive Electrochemical MUC1 Sensing Platform Based on Electroactive Cu-MOFs Decorated by AuPt Nanoparticles. Issue 8 (21st April 2020)

• Record Type: Journal Article
• Authors: Li, Ya; Hou, Lina; Liu, Zhiling; Lu, Wenbo; Zhao, Man; Xiao, He; Hu, Tianjun; Zheng, Zhanfeng; Jia, Jianfeng; Wu, Haishun
• Is Part Of: Journal of the Electrochemical Society. Volume 167:Issue 8(2020)
• Publication Date: 2020-04-21
• Subjects: Mucin 1 -- Cu-MOFs -- Au-Pt alloy nanoparticles -- Catalytic hairpin assembly -- Electrochemical sensing platform; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/1945-7111/ab88b9
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : Mucin 1 protein (MUC1) is a well-known tumor marker overexpressed in a variety of human malignancies, and thus its highly selective and sensitive detection is significantly desired for clinical diagnostics. Herein, a sensitive electrochemical MUC1 sensing platform has been fabricated based on copper-based metal-organic frameworks (Cu-MOFs) decorated with gold-platinum alloy nanoparticles (AuPt NPs). Besides serving as a redox probe to avoid introducing extra redox media, the electroactive Cu-MOFs function as catalysts in the hydrogen peroxide reduction, resulting in electrochemical signal amplification. Meanwhile, AuPt NPs loaded on the Cu-MOFs mimic horseradish peroxidase to promote the reduction of hydrogen peroxide, giving rise to the secondary-amplified electrochemical signal. Moreover, the target MUC1 triggered catalytic hairpin assembly (CHA) further improves the sensitivity of the developed biosensor via the target recycling amplification. The constructed sensing platform displayed a desirable dynamic range between 10 fM and 10 nM in the MUC1 detection, with a sufficiently low limit-of-detection (3.33 fM). The current strategy of utilizing hybrid nanocomposite consisting of MOFs and alloy NPs and enzyme-free CHA provides a promising platform for the detection of biomarkers in the clinical diagnosis with high selectivity and sensitivity.