BaP-metals co-exposure induced tissue-specific antioxidant defense in marine mussels Mytilus coruscus. (August 2018)

• Record Type: Journal Article
• Authors: Chen, Siyu; Qu, Mengjie; Ding, Jiawei; Zhang, Yifei; Wang, Yi; Di, Yanan
• Is Part Of: Chemosphere. Volume 205(2018)
• Page Start: 286
• Page End: 296
• Publication Date: 2018-08
• Subjects: Marine mussels -- Antioxidant system -- BaP-Metals co-exposure -- Tissue-specific; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.04.109
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Both benzo(α)pyrene (BaP) and metals are frequently found in marine ecosystem and can cause detrimental effects in marine organism, especially the filter feeder-marine mussels. Although the biological responses in mussels have been well-studied upon the single metal or BaP exposure, the information about antioxidant defense, especially in different tissues of mussels, are still limited. Considering the variety of contaminants existing in the actual marine environment, single BaP (56 μg/L) and the co-exposure with Cu, Cd and Pb (50 μg/L, 50 μg/L and 3 mg/L respectively) were applied in a 6 days exposure followed by 6 days depuration experiment. The alterations of superoxide dismutase (SOD), catalase (CAT) activities and total antioxidant capacity (TAC) level were assessed in haemolymph, gills and digestive glands of marine mussels, Mytilus coruscus . An unparalleled change in antioxidant biomarkers was observed in all cells/tissues, with the SOD activity showing higher sensitivity to exposure. A tissue-specific response showing unique alteration in gill was investigated, indicating the different function of tissues during stress responses. Depressed antioxidant effects were induced by BaP-metals co-exposure, indicating the interaction may alter the intact properties of BaP. To our knowledge, this is the first research to explore the antioxidant defense induced by combined exposure of BaP-metals regarding to tissue-specific responses in marine mussels. The results and experimental model will provide valuable information and can be utilized in the investigation of stress response mechanisms, especially in relation to tissue functions in marine organism in the future. Highlights: Antioxidant response was detected by measuring SOD, CAT activities and TAC level. BaP-metals induced diverse antioxidant defenses were firstly observed in mussels. Tissue- and contaminant-specific antioxidant responses were found under stresses. SOD activity can be a relatively sensitive biomarker to indicate oxidative stress. Effects induced by BaP can be depressed by co-exposure with metals.