Insights into disruption of lipid metabolism in digestive gland of female scallop Chlamys farreri under B[a]P exposure. (15th April 2022)

• Record Type: Journal Article
• Authors: Gao, Zhongyuan; Pan, Luqing; Xu, Ruiyi; Zhou, Yueyao; Li, Dongyu
• Is Part Of: Environmental pollution. Volume 299(2022)
• Publication Date: 2022-04-15
• Subjects: Benzo[a]pyrene -- Chlamys farreri -- Reproduction -- Transcriptome -- Lipid metabolism; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.118904
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Lipids are the main energy support during gametogenesis. Digestive gland is the key organ of aquatic animal metabolism for storing nutrition and supplying energy. It participates in a variety of life activities (such as growth, digestion, immunity, and reproduction). Nutrients stored in digestive glands, especially lipids, provide energy for reproductive behaviors such as gametogenesis and ovulation. A large number of studies have confirmed the accumulation of lipids from digestive gland to gonad during gametogenesis. At present, the research on the interference mechanism of persistent organic pollutants (POPs) on lipid metabolism of aquatic animals and the adaptive response of aquatic animals to POPs stress focus on biochemical levels or a few genes. The potential molecular mechanism of lipid metabolism interference needs to be further studied. In addition, as an important stage of aquatic animals, the reproductive period is a vigorous period of lipid metabolism. However, at present, there is no report on the molecular mechanism of POPs interfering with the lipid metabolism of the digestive gland in the reproductive process of aquatic animals. In this study, female scallop C. farreri was cultured in natural seawater and exposed to 4 μg/L B[a]P in seawater. Transcriptome analysis of digestive glands at multiple stages (proliferative stage, growth stage, mature stage and spawn stage) was performed, and iPath pathway analysis was used to analyze lipid metabolism pathways and differential genes. The interference mechanism of lipid metabolism in bivalves during reproductive period was revealed. This study will provide valuable genomic information on the role of digestive glands in lipid metabolism and reproduction of C. farreri, and will contribute to further functional genomics of bivalves and other closely related species. Graphical abstract: Image 1 Highlights: Lipid effects of B[a]P on scallops Chlamys farreri was studied. B[a]P caused lipid accumulation in digestive gland of reproductive female scallops. Disturbance of lipid metabolism in bivalves during reproductive stages. Function of mTOR pathway on lipid metabolism in bivalves have been discussed.