Lipid-mediated biosynthetic labeling strategy for in vivo dynamic tracing of avian influenza virus infection. (April 2022)

• Record Type: Journal Article
• Authors: Liu, Junfang; Su, Minhong; Chen, Xin; Li, Zhongli; Fang, Zekui; Yi, Li
• Is Part Of: Journal of biomaterials applications. Volume 36:Number 9(2022)
• Page Start: 1689
• Page End: 1699
• Publication Date: 2022-04
• Subjects: Biosynthetic labeling strategy -- avian influenza virus -- streptavidin-biotin conjugation -- dynamic visualization -- viral infection; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/08853282211063298
• Languages: English
• ISSNs: 0885-3282

Abstract

Monitoring the infection behavior of avian influenza viruses is crucial for understanding viral pathogenesis and preventing its epidemics among people. A number of viral labeling methods have been utilized for tracking viral infection process, but most of them are laborious or decreasing viral activity. Herein we explored a lipid biosynthetic labeling strategy for dynamical tracking the infection of H5N1 pseudotype virus (H5N1p) in host. Biotinylated lipids (biotinyl Cap-PE) were successfully incorporated into viral envelope when it underwent budding process by taking advantage of host cell-derived lipid metabolism. Biotin-H5N1p virus was effectively in situ–labeled with streptavidin-modified near-infrared quantum dots (NIR SA-QDs) using streptavidin-biotin conjugation with well-preserved virus activities. Dual-labeled imaging obviously shows that H5N1p viruses are primarily taken up in host cells via clathrin-mediated endocytosis. In animal models, Virus-conjugated NIR QDs displayed extraordinary photoluminescence, superior stability, and tissue penetration in lung, allowing us to long-term monitor respiratory viral infection in a noninvasive manner. Importantly, the co-localization of viral hemagglutinin protein and QDs in infected lung further conformed the dynamic infection process of virus in vivo. Hence, this in situ QD-labeling strategy based on cell natural biosynthesis provides a brand-new and reliable tool for noninvasion visualizing viral infection in body in a real-time manner.