A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea. (February 2022)

• Record Type: Journal Article
• Authors: Kwon, Sun-Jung; Cho, Young-Eun; Byun, Hee-Seong; Kwak, Hae-Ryun; Seo, Jang-Kyun
• Is Part Of: Molecular and cellular probes. Volume 61(2022)
• Publication Date: 2022-02
• Subjects: Tomato spotted wilt virus -- Resistance-breaking -- Molecular diagnosis -- Multiplex RT-PCR -- Pepper; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2022.101792
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Tomato spotted wilt virus (TSWV) is a highly destructive virus for pepper. Introgression of the resistance gene Tsw in pepper is used to manage TSWV worldwide; however, the occurrence of Tsw resistance-breaking (RB) variants threatens the pepper industry. Here, we developed a multiplex reverse-transcription PCR assay for detection of recently emerged Tsw RB variants in South Korea with high specificity and sensitivity. Highlights: A novel primer set for detection of Tsw RB variants of TSWV is described. The specificity and sensitivity of multiplex RT-PCR was tested using the primer set. The multiplex RT-PCR assay is rapid, accurate, and reliable for TSWV diagnosis.