Targeting and promoting atherosclerosis regression using hybrid membrane coated nanomaterials via alleviated inflammation and enhanced autophagy. (March 2022)

• Record Type: Journal Article
• Authors: You, Peidong; Mayier, Aziguli; Zhou, Hongyan; Yang, Anning; Fan, Jialong; Ma, Shengchao; Liu, Bin; Jiang, Yideng
• Is Part Of: Applied materials today. Volume 26(2022)
• Publication Date: 2022-03
• Subjects: Atherosclerosis -- Atorvastatin -- Biomimetic nanomaterials -- Inflammation -- Autophagy; AT atorvastatin -- GOQD graphene oxide quantum dots -- Møm macrophages membrane -- RBCm red blood cell membrane -- [Mø+RBC]m M, Mø-RBC hybrid membrane -- HA hyaluronic acid -- GP NPs GOQDs-PEG nanomaterials -- LPS lipopolysaccharide -- oxLDL oxidized low-density lipoprotein -- HFD high-fat diet -- PLGA poly (lactic-co-glycolic acid) copolymer -- RES reticuloendothelial system -- MTT thiazolyl blue tetrazolium bromide -- NHS N-hydroxy-uccinimide -- EDC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide -- ORO Oil Red O -- BCA bicinchoninic acid DiD, 1, 1-dioctadecyl-3, 3, 3, 3-tetramethylin-dodicarbocyanine iodide -- DiI 1, 1′-dioctadecyl-3, 3, 3′, 3′-tetramethylin-docarbocyanine perchlorate -- DiO 3, 3′dioctadecyloxacarbocyanine perchlorate -- PVDF polyvinylidene difluoride -- SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- FRET Förster resonance energy transfer -- FT-IR Fourier transform infrared spectroscopy -- DLS Dynamic light scattering -- TEM transmission electron microscope -- CLSM confocal laser scanning microscope -- H&E hematoxylin-eosin -- RAW264.7 mouse macrophage cell line -- HUVECs human umbilical vein endothelial cells -- VSMC vascular smooth muscle cell line -- HL7702 human liver cell line -- MPC5 mouse podocyte cell lines -- H9C2 rat cardiac myoblast cell line -- ROS reactive oxygen species -- MCP-1 monocyte chemoattractant protein-1 -- TNF-α tumor necrosis factor-α -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- ABCA1 ATP-binding cassette transporter A1 -- ABCG1 ATP-binding cassette transporter G1 -- α-SMA α-smooth muscle actin -- ICAM-1 intercellular cell adhesion molecule-1 -- SR-BI scavenger receptor BI -- MMP-9 matrix metalloproteinase-9 -- RBC red blood cells -- WBC white blood cells -- PLT platelets -- CRP C-reactive protein -- HGB hemoglobin -- TP total Protein -- ALB albumin -- GLOB globulin -- ALT alanine aminotransferase -- AST aspartate transaminase -- UA blood urea -- UN urea nitrogen -- TG total cholesterol -- TC triglyceride -- HDL high density lipoprotein -- LDL low density lipoprotein; Materials science -- Periodicals; Materials -- Research -- Periodicals; 620.1105
• DOI: 10.1016/j.apmt.2022.101386
• Languages: English
• ISSNs: 2352-9407

Abstract

Highlights: Hybrid membrane coated nanomaterials increase the accumulation of drugs in plaques. HA modification improve nanomaterial's uptake efficiency in activated macrophages. HA-M@AT@GP inhibited the early atherosclerosis by alleviating inflammation. HA-M@AT@GP regressed the advanced atherosclerosis by enhancing autophagy. ABSTRACT: Atherosclerosis is the leading cause of death and disability worldwide, which is lack of efficient strategy for targeted therapy. In this work, we reported a hybrid membrane coated graphene oxide quantum dots (GOQDs) nanomaterial loading atorvastatin (AT) to achieve targeted atherosclerosis therapy. In this biomimetic nanomaterial, macrophages membrane (Møm) was used to extend the half-life of AT and enhance the accumulation of AT in the atherosclerotic plaques. Meanwhile, red blood cell membrane (RBCm) was adopted to reduce the amount of Møm usage. Furthermore, hyaluronic acid (HA) was inserted into the hybrid membrane to enhance the internalization of nanomaterial into activated macrophages of atherosclerotic plaques. In vitro assay demonstrated that this nanomaterial can release AT in an acidic pH-responsive manner. In vivo assay demonstrated that both long-term low-dose and short-term high-dose regimens with favorable safety can effectively inhibit the development of the early atherosclerosis and markedly regress the progression of advanced atherosclerotic plaques, respectively. Molecular mechanism exploration indicated that the anti-atherosclerosis effect of the nanomaterial was mediated by alleviating inflammation to reduce lipid influx and enhancing autophagy to promote cholesterol efflux. Overall, this biomimetic nanomaterial provides an alternative for developing safe and efficient strategy for atherosclerosis therapy.