XT-type DNA hydrogels loaded with VEGF and NGF promote peripheral nerve regeneration via a biphasic release profile. (5th November 2021)

• Record Type: Journal Article
• Authors: Liu, Songyang; Liu, Yijun; Zhou, Liping; Li, Ci; Zhang, Meng; Zhang, Fengshi; Ding, Zhentao; Wen, Yongqiang; Zhang, Peixun
• Is Part Of: Biomaterials science. Volume 9:Number 24(2021)
• Page Start: 8221
• Page End: 8234
• Publication Date: 2021-11-05
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d1bm01377g
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Preparation of XT-type DNA hydrogel loaded with VEGF and NGF which could achieve their biphasic release.The repair effect and mechanism of the XT-type DNA hydrogels on a 10 mm peripheral nerve defect were investigated in vitro and in vivo . Abstract : Peripheral nerve injury (PNI) remains an unresolved challenge in the medicine area. With the development of biomaterial science and tissue engineering, a variety of nerve conduits were widely applied for repairing long defect PNI. DNA materials are developing rapidly due to their multiple advantages. In the present study, we aim to combine a DNA hydrogel, vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) to construct a new type of delivery system, which could achieve a biphasic release profile of VEGF and NGF by taking advantage of the different degradation rates between X- and T-type DNA. In vitro results showed that the DNA gel + VEGF/NGF system could promote proliferation, migration and myelination of Rat Schwann cells (RSC) while maintaining cell viability. In vivo results indicated a better effect of DNA gel + VEGF/NGF on promoting repair of long defect PNI than the hollow chitin conduits (CT), DNA gel or VEGF/NGF group. The new technology invention holds promising clinical application prospects for repairing PNI and may be used broadly after step-by-step improvement.