Biodegradable hyaluronic acid-based, nitric oxide-releasing nanofibers for potential wound healing applications. (3rd November 2021)

• Record Type: Journal Article
• Authors: Gwon, Kihak; Choi, Won Il; Lee, Seonhwa; Lee, Jin Sil; Shin, Jae Ho
• Is Part Of: Biomaterials science. Volume 9:Number 24(2021)
• Page Start: 8160
• Page End: 8170
• Publication Date: 2021-11-03
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d1bm01019k
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : The biodegradable and nitric oxide (NO)-releasing hyaluronic acid (HA)-based nanofibers developed in this study have the potential application in wound healing. Abstract : Nitric oxide (NO) is one of the smallest gas molecules with pharmaceutical and potential wound therapeutic effects due to its ability to regulate inflammation and eradicate bacterial infections. Recently, NO-releasing synthetic polymer-based nanofibers have become promising candidates for wound healing due to their facile functionalisation, tunable mechanical properties, and large effective surface areas. However, synthetic polymer-based nanofibers suffer from poor degradability in the physiological milieu, which restricts their use in in vivo applications. In this study, we developed biodegradable and nitric oxide-releasing nanofibers for potential wound healing applications. We synthesised dual-functionalised hyaluronic acid (HA) containing methacrylate groups and N -diazeniumdiolate (NONOate)-NO donor groups and capable of forming crosslinked, electrospun nanofibers, with an effective NO payload, through an electrospinning process and photoinitiated polymerisation. Nuclear magnetic resonance, Fourier transform infrared spectroscopy, and ultraviolet-visible spectroscopy confirmed the successful synthesis of the functionalised HA. Control over both the NO donor and HA concentrations allowed for the preparation of NO-releasing, HA-based nanofibers of varying diameters (240–490 nm), NO payloads (10–620 nmol mg −1 ), maximum amounts of NO released (160–8920 ppb mg −1 ), and NO release durations (1.5–20.2 h). Moreover, the NO-releasing nanofibers had good biodegradability and potential wound healing effects without any observed cytotoxicity. The biodegradable and NO-releasing HA-based nanofibers developed in this study have the potential application in wound healing.