Crosslinked Poly(N‐Isopropylacrylamide)‐Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment. Issue 23 (6th November 2019)

• Record Type: Journal Article
• Authors: Konishi, Tomomi; Mizutani Akimoto, Aya; Nishimoto, Taihei; Tokura, Yuki; Tenjimbayashi, Mizuki; Homma, Kenta; Matsukawa, Ko; Kaku, Taisei; Hiruta, Yuki; Nagase, Kenichi; Kanazawa, Hideko; Shiratori, Seimei
• Is Part Of: Macromolecular rapid communications. Volume 40:Issue 23(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-11-06
• Subjects: fast cell detachment -- microfibers -- thermoresponsive polymers -- volume expansion; Macromolecules -- Periodicals; Polymers -- Periodicals; Chemistry -- Periodicals; 547.705
• DOI: 10.1002/marc.201900464
• Languages: English
• ISSNs: 1022-1336

Abstract

Abstract: Stimuli‐responsive smart materials are a key to the realization of next‐generation medical technologies. Among them, the temperature‐responsive polymer poly( N ‐isopropylacrylamide) (PNIPAAm) is attracting particular attention because it is easy to use in physiological conditions. PNIPAAm‐grafted surfaces can undergo temperature‐modulated cell adhesion and detachment without proteolytic enzymes, and can be used as cell‐separating materials through selective cell adhesion/detachment. However, cell detachment at reduced temperatures is problematic because it takes several hours. A novel thermoresponsive crosslinked microfiber system that can greatly reduce the cell detachment time is introduced in this study. The crosslinked fibers provide temperature‐dependent volume change, and enable cell detachment within 10 min of reducing the temperature, which is one‐sixth of the time required in previous studies. The prompt cell detachment is thought to arise from a completely new mechanism derived from fiber swelling. This system will make a significant contribution as a novel cell manipulating system for next‐generation medical technology. Abstract : Temperature‐dependent, highly responsive cell adhesion/detachment control is achieved using poly( N ‐isopropylacrylamide)‐based crosslinked microfibers. The fibers facilitate stable cell adhesion at 37 °C, and detachment within 10 min of reducing the temperature to 25 °C. The prompt cell detachment is thought to result from temperature‐dependent fiber swelling. This system could significantly contribute to biomedical technologies.