Antimicrobial resistance profiling and burden of resistance genes in zoonotic Salmonella isolated from broiler chicken. Issue 1 (2nd October 2021)

• Record Type: Journal Article
• Authors: Das, Tridip; Rana, Eaftekhar Ahmed; Dutta, Avijit; Bostami, Md. Bayazid; Rahman, Mizanur; Deb, Probir; Nath, Chandan; Barua, Himel; Biswas, Paritosh Kumar
• Is Part Of: Veterinary medicine and science. Volume 8:Issue 1(2022)
• Page Start: 237
• Page End: 244
• Publication Date: 2021-10-02
• Subjects: antimicrobial resistance -- chicken -- resistance genes -- Salmonella; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.648
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Background: Salmonella is frequently found in poultry of which only motile serovars have zoonotic significance due to their potential to induce human gastrointestinal infections. Antimicrobial resistance, being a public health concern, the emergence of multidrug‐resistant (MDR) Salmonella serotypes affecting food chain has greater impact worldwide. Aim: Information on circulation of zoonotic Salmonella strains in commercial poultry farm level is limited in many parts of the world. This cross‐sectional study was aimed to investigate the zoonotic Salmonella strains circulating in the broiler farm environment with their detailed antimicrobial resistance profiling. Methods: Pooled faecal samples were collected randomly from commercial broiler farms of Chattogram district, Bangladesh. Standard bacteriological procedure was followed to isolate Salmonella, and identification was confirmed by genus specific polymerase chain reaction (PCR). After phenotypic characterisation of resistance profile against eight antimicrobials by disc diffusion technique, all strains were screened by PCR for some selected resistance genes. Results: Out of the 350 samples, Salmonella was isolated and identified from 86 samples. In antimicrobial sensitivity testing, more than 98.8% isolates showed resistance to ampicillin and 94.2% to tetracycline followed by enrofloxacin (56%) and ciprofloxacin (50%). Notably, 94% isolates were found to be MDR. The results of PCR assays revealed that 81.4% of the isolates were carrying the tetA gene, 19.8% the tetB and 10.47% the tetC gene. The prevalence of the isolates bearing the bla TEM, bla CTX‐M and Sul ‐I gene were 95.4%, 7.0 % and 37.2 %, respectively. Conclusion: There is a great risk to secure healthy poultry products due to the circulation of these MDR zoonotic Salmonella Abstract : This cross‐sectional study was aimed to investigate the zoonotic Salmonella strains circulating in the broiler farm environment with their detailed antimicrobial resistance profiling. Out of the 350 samples, prevalence of Salmonella was 24% and multidrug‐resistant (MDR) Salmonella was 94%. There is a great risk to secure healthy poultry products due to the circulation tetA, tetB, tetC, bla TEM, bla CTX‐M and Sul ‐I antimicrobial resistance genes in these MDR zoonotic Salmonella .