Multifunctional crosslinkable itaconic acid copolymers for enzyme immobilization. (May 2018)

• Record Type: Journal Article
• Authors: Müller, Felix; Torger, Bernhard; Allertz, Peter J.; Jähnichen, Klaus; Keßler, Stefan; Müller, Martin; Simon, Frank; Salchert, Katrin; Mäurer, Haike; Pospiech, Doris
• Is Part Of: European polymer journal. Volume 102(2018)
• Page Start: 47
• Page End: 55
• Publication Date: 2018-05
• Subjects: ATR-FTIR spectroscopy -- Crosslinking -- Enzyme-immobilization -- Itaconic acid -- Methacrylate copolymer; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2018.03.014
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Crosslinkable, enzymatically active coatings for water treatment to remove phenolic trace substances. Highlights: New MMA terpolymers provide multifunctionality embedded in one macromolecule. UV-crosslinked films are hydrolytically and mechanically stable. Itaconic acid comonomer allows to fix enzymes covalently – proven by ATR-FTIR. The copolymers form a suitable alternative to poly(ethene-alt-maleic anhydride). Immobilized Laccase degrades phenolic compounds (diclofenac, bisphenol-A). Abstract: UV-Crosslinkable itaconic copolymers are developed to provide new multifunctional materials for coatings which combine crosslinkable functionalities and the possibility to immobilize enzymes. The polymer-immobilized enzymes were used for water treatment to decompose persistent organic molecules. Introduction of suitable comonomers allows tailoring the mechanical and chemical properties for special applications. Copolymers containing MMA and itaconic anhydride were chosen because of the formation of long-term stable anhydride functionalities. These anhydride functionalities are employed to attach enzymes covalently. 4-Benzoylphenyl methacrylate is used as comonomer for UV-initiated crosslinking. Terpolymers are successfully obtained by radical copolymerization in solution. The copolymers are compared to poly(ethylene- alt -maleic anhydride) [P(EMA)] often used with respect to enzyme immobilization, activity and hydrolytic stability. The hydrolysis stability of the copolymers against water is studied by ATR-FTIR spectroscopy. Thin films are prepared on glass substrates in a layer-by-layer procedure by spin-coating. The layer formation is monitored by ATR-FTIR spectroscopy. UV-crosslinking of the copolymer films is performed taking the optimal irradiation dose that avoids polymer degradation. ATR-FTIR spectroscopy verifies the coupling reaction between amino groups of the enzyme and the anhydride groups on the surface of the crosslinked polymer film. The syringaldazine (4-hydroxy-3, 5-dimethoxybenzaldehyde azine) test and 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay demonstrate that the immobilized enzymes maintain their activities. The functional copolymers showed a significant effect in reduction of persistent organic pollutants in contaminated waste water.