Light emitting diode (LED) therapy reduces local pathological changes induced by Bothrops asper snake venom. (15th September 2018)

• Record Type: Journal Article
• Authors: Campos, Gabriela Russo Soeiro; de Moura, Kátia Margareth Bitton; Barbosa, Ana Maria; Zamuner, Luis Fernando; Nadur-Andrade, Nikele; Dale, Camila Squarzoni; Gutiérrez, José María; Chavantes, Maria Cristina; Zamuner, Stella Regina
• Is Part Of: Toxicon. Volume 152(2018)
• Page Start: 95
• Page End: 102
• Publication Date: 2018-09-15
• Subjects: Bothrops asper venom -- Edema -- Hyperalgesia -- Light emitting diode -- LED therapy myonecrosis; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2018.07.029
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The therapeutic effect of the Light Emitting Diode (LED) treatment in two wavelengths (635 or 945 nm) was evaluated in the local pathological alterations induced by Bothrops asper snake venom. Mice received irradiation of infrared LED (120 mW, 945 nm) or red LED (110 mW, 635 nm) applied immediately, 1 and 2 h after venom injection. LED treatment reduced edema formation in the plantar region and gastrocnemius muscle and significantly reduced neutrophil migration and hyperalgesia after the venom injection. Also, both infrared LED and red LED treatment significantly reduced myonecrosis, as revealed by muscle CK and plasma CK levels. Histological analysis corroborated the reduction in the extent of venom-induced myonecrosis. In conclusion, our data demonstrates that PBM with LED light in both red and infrared wavelengths, when applied after envenomation in mice, reduces the extent of myotoxicity, edema, inflammatory infiltrate and hyperalgesia, suggesting that photobiomodulation is a potential therapeutic approach that should be further investigated for the treatment of local effects of Bothrops snakebite. Highlights: Bothrops asper venom causes prominent local pathological and inflammatory effects. Antivenom therapy does not neutralize the local effects caused by Bothrops asper venom. LED therapy reduces local effects caused by Bothrops asper venom. LED is a promising tool in the treatment of local envenomation in snakebites.