Honey protects against wings posture error and molecular changes related to mitochondrial pathways induced by hypoxia/reoxygenation in adult Drosophila melanogaster. (1st August 2018)

• Record Type: Journal Article
• Authors: Cruz, L.C.; Ecker, A.; Rodrigues, N.R.; Martins, I.K.; Posser, T.; Maciel, F.E.; Vargas, M.A.; Barbosa, N.V.; Franco, J.L.
• Is Part Of: Chemico-biological interactions. Volume 291(2018)
• Page Start: 245
• Page End: 252
• Publication Date: 2018-08-01
• Subjects: Hypoxia -- Honey -- Wing posture -- Ewg -- opa1-like -- Mitochondria; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2018.06.033
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: We conducted an investigation to evaluate the effects of Brazilian Pampa biome honey and its major phenolic compounds on the development of an erected wings posture phenotype and related mitochondrial aspects induced by Hypoxia/Reoxygenation (H/R) in Drosophila melanogaster . Flies were pre-treated for 3 days with a 10% honey solution and different concentrations of caffeic acid and ρ-coumaric acid and then submitted to hypoxia for 3 h. We observed that after reoxygenation, some flies acquired an erected wings posture and that this feature may be related to mortality. In addition, H/R induced down-regulation of e wg mRNA expression, which could be associated to the observed complex phenotype. H/R also caused a dysregulation in opa1-like, ldh and diap genes expression and reduced O2 fluxes in flie's mitochondria. Honey mitigated opa1-like mRNA expression changes provoked by H/R. Differently from honey, caffeic and ρ-coumaric acids displayed no protective effects. In conclusion, we report for the first time the protective effects of honey against complex phenotypes and mitochondrial changes induced by H/R in adult flies. Highlights: Hypoxia/Reoxygenation (H/R) induced a erected wings posture in D. melanogaster . Honey treatment decreased the erected wings posture frequency induced by H/R. H/R induced down-regulation of e wg mRNA expression. Locomotor activity, wings posture and ewg - opa1-like genes post H/R may be linked. Honey beneficial effects possibly involve antioxidant and anti-apoptotic properties.