Fluorogenic l-alanylaminopeptidase substrates derived from 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins and their potential applications in diagnostic microbiology. Issue 10 (15th May 2019)

• Record Type: Journal Article
• Authors: Cellier-Rastit, Marie; James, Arthur L.; Orenga, Sylvain; Perry, John D.; Robinson, Shaun N.; Turnbull, Graeme; Stanforth, Stephen P.
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 29:Issue 10(2019)
• Page Start: 1227
• Page End: 1231
• Publication Date: 2019-05-15
• Subjects: Fluorogenic substrates -- l-Alanylaminopeptidase -- Microorganism detection -- Quinolines -- Coumarins; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2019.03.011
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Six novel fluorogenic enzyme substrates for detecting l -alanylaminopeptidase activity in microorganisms have been prepared and evaluated in Columbia agar media. The substrates are l -alanyl derivatives of 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins. Both the quinoline and coumarin series of substrates produced fluorescence in the presence of Gram-negative microorganisms. In contrast, fluorescence generation in the presence of the Gram-positive microorganisms and yeasts was limited or absent.