HPLC-DAD fingerprinting analysis, antioxidant activities of Tithonia diversifolia (Hemsl.) A. Gray leaves and its inhibition of key enzymes linked to Alzheimer's disease. (2018)

• Record Type: Journal Article
• Authors: Ojo, Oluwafemi Adeleke; Ojo, Adebola Busola; Ajiboye, Basiru Olaitan; Olaiya, Oluranti; Okesola, Mary Abiola; Boligon, Aline Augusti; de Campos, Marli Matiko Anraku; Oyinloye, Babatunji Emmanuel; Kappo, Abidemi Paul
• Is Part Of: Toxicology reports. Volume 5(2018)
• Page Start: 585
• Page End: 592
• Publication Date: 2018
• Subjects: Tithonia diversifolia leaves -- Antioxidant activity -- Cholinergic enzymes -- Alzheimer's disease; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2018.05.003
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Current study supports antioxidant activity of Tithonia diversifolia leaves. Current study also supports anti cholinesterase activity of Tithonia diversifolia leaves. Current investigation adequately characterized the phenolic compounds of Tithonia diversifolia leaves. Abstract: Tithonia diversifolia (Hemsl.) A. Gray leaves have long been used to manage neurodegenerative diseases without scientific basis. This study characterized the phenolic constituents, evaluated the antioxidant properties of phenolic extracts from T. diversifolia leaves used as traditional medicine in Africa and its inhibition of key enzymes linked to Alzheimer's disease. The extract was rich in phenolic acids (gallic acid, chlorogenic acid, caffeic acid and p -coumaric acid) and flavonoids (apigenin) and had 1, 1-diphenyl-2-picryl-hydrazil radical scavenging abilities (IC50 = 41.05 μg. mL −1 ), 2, 2-Azino-bis3-ethylbenthiazoline-6sulphonic acid radical scavenging ability (IC50 = 33.51 μg. mL −1 ), iron chelation (IC50 = 38.50 μg. mL −1 ), reducing power (Fe 3+ - Fe 2+ ) (7.34 AAEmg/100 g), inhibited acetylcholinesterase (IC50 = 39.27 μg mL −1 ) and butyrylcholinesterase (IC50 = 35.01 μg mL −1 ) activities. These results reveal the leaf as a rich source of phenolic compounds with antioxidant and cholinesterase inhibitory activity.