Dual-drug loaded Janus graphene oxide-based thermoresponsive nanoparticles for targeted therapy. (25th April 2018)

• Record Type: Journal Article
• Authors: Khoee, Sepideh; Karimi, Mohammad Reza
• Is Part Of: Polymer. Volume 142(2018)
• Page Start: 80
• Page End: 98
• Publication Date: 2018-04-25
• Subjects: Graphene oxide -- Janus nanoparticles -- Microemulsion -- Dual-drug delivery -- Thermoresponsive polymer; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2018.03.022
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: Graphene oxide (GO) is extensively recognized as an effective material in a variety of fields and specifically for various biomedical applications. However, the modification of each side of a GO sheet, needed for preparation of heterogeneous nanoparticles, is yet hypothetical and unclear. Here, we have developed a new strategy for the synthesis of two-faced GOs by simultaneous anchoring of different polymers with different hydro-affinity via Pickering emulsion (two-phase media). Alkynyl-modified GO was prepared for unsymmetrically attaching azidated-poly(ɛ-caprolactone) (PCL) and ( N -isopropylacrylamide- co -acrylamide- co -allylamine) terpolymer to the surface of GO through click reaction. During this reaction, a thermoresponsive nanoparticles with specific morphology, known as " Janus " nanostructure, will be produced. PCL/terpolymer homostructure was also fabricated via solution (one-phase) reaction, where two types of polymers were grafted on the both surfaces of GO disorderly, named " mixed " nanoparticles. In the following, drug loaded nanocarriers were synthesized by simultaneously using quercetin and 5-FU as hydrophobic and hydrophilic drugs. The bioapplicability of these nanoparticles were evaluated by comparison of their in vitro release profiles and cell cytotoxicity at two temperatures of 37 and 40 °C. Graphical abstract: Image 1 Highlights: PNIPAM/PCL grafted graphene oxide was prepared as smart nanocarrier. Pickering emulsion was used for synthesis of two-faced Janus nanoparticles. For comparison mixed morphology of this type of nanocarrier was also synthesized. 5-FU and Quercetin as two drugs with different hydroaffinity were loaded. In-vitro release profiles and cell cytotoxicity of nanoparticles were studied.