Carob pulp syrup: A potential Mediterranean carbon source for carotenoids production by Rhodosporidium toruloides NCYC 921. (September 2018)

• Record Type: Journal Article
• Authors: Martins, Vasco; Dias, Carla; Caldeira, João; Duarte, Luís C.; Reis, Alberto; Lopes da Silva, Teresa
• Is Part Of: Bioresource technology reports. Volume 3(2018)
• Page Start: 177
• Page End: 184
• Publication Date: 2018-09
• Subjects: Carob pulp syrup -- Rhodosporidium toruloides -- Carotenoids -- Flow cytometry -- Cell viability; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2018.07.008
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: Carob pulp syrup (CPS) was used as carbon source to produce carotenoids from Rhodosporidium toruloides . To increase the carbon concentration in the growth medium aiming at the carotenoid production improvement, the CPS was concentrated and two different total sugar (TS) concentrations (195.6 g/L and 548.7 g/L) were studied. CPS 195.6 g/L TS contained 4.1 g/L and 0.7 g/L of hydroxymethyl furfural (HMF) and furfural, respectively. CPS 548.7 g/L TS contained 17.7 g/L of HMF and 1.2 g/L of furfural, respectively. >42% of metabolically active cells (with intact membrane and enzymatic activity) were detected throughout the course of the yeast cultivation on CPS 195.6 g/L TS. On the contrary, the proportion of metabolically active cells was always below 28% during the yeast cultivation on CPS 548.7 g/L TS. Nevertheless, the maximum carotenoid content and productivity (0.42 mg/g and 0.43 mg/Lh, respectively) were obtained when using CPS 548.7 g/L TS. Highlights: Carob pulp syrup (CPS) was used as carbon source in media formulation. CPS solutions with 195.0 and 548.7 g/L of total sugars were tested for yeast growth. Concentrated CPS (548.7 g/L) induced the highest stress level in the yeast cells. Concentrated CPS induced the highest biomass and carotenoids yeast production.