Multiplasmid DNA in agarose gel electrophoresis: Suitable dye and temperature is essential for prominent profiles. Issue 3 (20th October 2021)

• Record Type: Journal Article
• Authors: Jiao, Qiuxia; Xin, Zhao; Gou, Qiufeng; Cao, Kang; Pan, Qu
• Is Part Of: Electrophoresis. Volume 43:Issue 3(2022)
• Page Start: 472
• Page End: 476
• Publication Date: 2021-10-20
• Subjects: Electrophoresis -- Gelred -- Goldview -- Multiplasmid DNA -- Temperature; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.202100206
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Nucleic acids dye Goldview is widely used in agarose gel electrophoresis (AGE). However, in this study, a sample of multiplasmid DNA (multi‐pDNA) stained with Goldview analyzed by AGE showed its instability at low temperature. Three types of DNA samples were analyzed, including linear DNA (ladder), single‐plasmid DNA (single‐pDNA), and multi‐pDNA, electrophoretic conditions were optimized by adjusting the dye, the buffer, and the temperature (1–50°C). The results showed that the light intensity of Gelred is 2.2‐times higher than that of Goldview in staining multi‐pDNA. Compared with the single‐pDNA and the linear DNA, the multi‐pDNA stained with Goldview was greatly affected by temperature. This short communication indicated that Gelred is a highly applicable dye for analyzing multiplasmid samples. The degree and the way of binding of Goldview to multi‐pDNA are greatly affected by temperature.