A dibenzoylmethane derivative inhibits lipopolysaccharide-induced NO production in mouse microglial cell line BV-2. (October 2018)

• Record Type: Journal Article
• Authors: Takano, Katsura; Ishida, Natsumi; Kawabe, Kenji; Moriyama, Mitsuaki; Hibino, Satoshi; Choshi, Tominari; Hori, Osamu; Nakamura, Yoichi
• Is Part Of: Neurochemistry international. Volume 119(2018)
• Page Start: 126
• Page End: 131
• Publication Date: 2018-10
• Subjects: Dibenzoylmethane -- Microglia -- NO production -- Cytokines; CBB coomassie brilliant blue -- DAN 2, 3-diaminonaphthalene -- DBM dibenzoylmethane -- DMEM Dulbecco's modified Eagle medium -- DMSO dimethylsulfoxide -- ER endoplasmic reticulum -- FBS fetal bovine serum -- IL interleukin -- iNOS inducible nitric oxide synthase -- LPS lipopolysaccharide -- MTT 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide -- NF-κB nuclear factor-kappa B -- PD Parkinson's disease -- RT-PCR reverse transcription-polymerase chain reaction -- TNF tumor necrosis factor; Neurochemistry -- Periodicals; Neurochemistry -- Periodicals; Neurochimie -- Périodiques; Neurochemistry; Periodicals; 612.804205
• DOI: 10.1016/j.neuint.2017.04.002
• Languages: English
• ISSNs: 0197-0186

Abstract

Abstract: Microglial activation has been suggested to play important roles in various neurodegenerative diseases by phagocytosis and producing various factors such as nitric oxide (NO), proinflammatory cytokines. Excessive production of NO, as a consequence of increased inducible nitric oxide synthase (iNOS) in microglia, contributes to the neurodegeneration. During a search for compounds that regulate endoplasmic reticulum (ER) stress, a dibenzoylmethane derivative, 2, 2'-dimethoxydibenzoylmethane (DBM 14–26) was identified as a novel neuroprotective agent (Takano et al., Am. J. Physiol. Cell Physiol . 293, C1884-1894, 2007). We previously reported in cultured astrocytes that DBM 14–26 protected hydrogen peroxide-induced cell death and inhibited lipopolysaccharide (LPS)-induced NO production (Takano et al., J. Neurosci. Res . 89, 955–965, 2011). In the present study, we assessed the effects of DBM 14–26 on microglia using the mouse cell line BV-2 and found that DBM 14–26 inhibited LPS-induced iNOS expression and NO production also in microglia. DBM 14–26 also suppressed LPS-induced IL-1β expression. Conditioned medium of BV-2 cells stimulated by LPS significantly decreased cell viability of neuron (human neuroblastoma SH-SY5Y cells) compared with the absence of LPS. Conditioned medium of BV-2 cells stimulated by LPS in the presence of DBM 14–26 did not significantly decreased cell viability of neuron. These results indicate that microglial activation by LPS causes neuronal cell death and DBM 14–26 protect neuron through the inhibition of microglial activation. Functional regulation of microglia by DBM 14–26 could be a therapeutic candidate for the treatment of neurodegenerative diseases. Highlights: Regulation of cellular functions of microglia by a dibenzoylmethane derivative (DBM). DBM inhibits LPS-induced iNOS expression and NO production. LPS-induced IL-1β mRNA is suppressed by DBM. DBM prevents the decrease of neuronal cell viability by incubation in LPS-stimulated microglial conditioned medium. Inhibition of microglial activation may be involved in neuroprotective effects by DBM.