28-day inhalation toxicity of 3-methoxybutyl chloroformate in rats. (2018)

• Record Type: Journal Article
• Authors: Kim, Hyeon-Yeong; Cho, Eun-Sang
• Is Part Of: Toxicology reports. Volume 5(2018)
• Page Start: 213
• Page End: 219
• Publication Date: 2018
• Subjects: ANOVA analysis of variance -- CT computed tomography -- EDTA ethylenediamine tetraacetic acid -- GHS Globally Harmonized System of Classification and Labelling of Chemicals -- GLP Good Laboratory Practice -- HCT hematocrit -- HGB hemoglobin concentration -- MCH mean corpuscular hemoglobin -- MCHC mean corpuscular hemoglobin concentration -- MCV mean corpuscular volume -- MNPCE micronucleated polychromatic erythrocytes -- MOE The Ministry of Environment -- NCE normochromatic erythrocytes -- NOAEL no observed adverse effect level -- OECD Organization for Economic Cooperation and Development -- PCE polychromatic erythrocytes -- PLT platelets -- RBC red blood cell counts -- RDW red cell distribution width -- REACH Registration, Evaluation, Authorization and Restriction of Chemicals -- SD Sprague-Dawley -- SPF specific-pathogen-free -- WBC white blood cell counts -- 3-MBCF 3-methoxy butyl chloroformate; 3-Methoxybutyl chloroformate -- Inhalation toxicity -- occupational hazard -- Sprague Dawley rats; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2017.12.007
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: 3-MBCF up to 6 ppm induced mortality in 28-day inhalation study of rats. The NOAEL of 3-MBCF in 28 day inhalation toxicity study, was less than 3 ppm. 3-MBCF under 12 ppm did not induce micronucleus formation in the bone marrow of rats. Abstract: The 28-day repeated inhalation study was applied for hazard assessment of 3-methoxybutyl chloroformate (3-MBCF) in Sprague Dawley rats. Groups of five rats per sex were exposed 6 h/day, 5 days per week for 4 weeks to test substance concentration (ranging from 3 to 12 ppm) using a whole-body exposure system. At the terminal sacrifice, following blood collection and gross pathological examination, organ weights were determined and fixed organs were examined. The micronucleus test was performed using bone marrow cells. Exposure of 3-MBCF induced mortality at concentrations above 6 ppm. Decreases in body weight and food intake, hematologic alterations, organ weight changes, and gross and microscopic findings were seen even at the lowest concentrations of 3 ppm. Histopathology revealed principal test substance exposure correlated with lesions in the respiratory tract in both male and female rats above 3 ppm. Groups of male rats exposed above 6 ppm show microscopic lesions in spleens, livers, testes and epididymides; however, the micronucleated polychromatic erythrocytes frequency in bone marrow cells was not changed. Based on histopathology of the respiratory tract and other organs, the no observed adverse effect level (NOAEL) of 3-MBCF in the present study was less than 3 ppm.