The effects of laser patterning 10CeTZP-Al2O3 nanocomposite disc surfaces: Osseous differentiation and cellular arrangement in vitro. Issue 8 (1st June 2018)

• Record Type: Journal Article
• Authors: Goyos-Ball, Lidia; Prado, Catuxa; Díaz, Raquel; Fernández, Elisa; Ismailov, Arnold; Kumpulainen, Tero; Levänen, Erkki; Torrecillas, Ramón; Fernández, Adolfo
• Is Part Of: Ceramics international. Volume 44:Issue 8(2018)
• Page Start: 9472
• Page End: 9478
• Publication Date: 2018-06-01
• Subjects: Ceramic nanocomposite -- Alumina -- Zirconia -- Laser patterning -- Osseous differentiation -- Cellular arrangement; Ceramics -- Periodicals; Céramique industrielle -- Périodiques; Ceramics; Periodicals; Electronic journals; 666
• DOI: 10.1016/j.ceramint.2018.02.164
• Languages: English
• ISSNs: 0272-8842

Abstract

Abstract: Customized square grid arrangements of different groove depths (1.0, 1.5 and 3.0 µm) and separations (10 and 30 µm) were successfully laser patterned, using a nanosecond pulsed fibre laser, on the surface of 10 mol% ceria-stabilized zirconia and alumina (10CeTZP-Al2 O3 ) nanocomposite discs (diameter: 10 mm; thickness: 1.5 mm). The patterned surfaces and the in vitro biological response of osteoblasts (SAOS-2) towards them were thoroughly analysed. In terms of composition, the laser treatment was found to cause superficial monoclinic-tetragonal zirconia phase transformation and alumina evaporation. In vitro, the most effective grid configuration for osseous differentiation was found to be 1.5 µm groove depth and 10 µm groove separation, and confocal microscopy revealed that the cells show a tendency to be sorted as groove depth increases. It is thought that custom-made patterns could be produced to guide cell attachment in vivo, which could favour implant integration and reduce healing time.