Genome engineering for microbial natural product discovery. (October 2018)

• Record Type: Journal Article
• Authors: Choi, Si-Sun; Katsuyama, Yohei; Bai, Linquan; Deng, Zixin; Ohnishi, Yasuo; Kim, Eung-Soo
• Is Part Of: Current opinion in microbiology. Volume 45(2018)
• Page Start: 53
• Page End: 60
• Publication Date: 2018-10
• Subjects: Microbiology -- Periodicals; 579.05
• DOI: 10.1016/j.mib.2018.02.007
• Languages: English
• ISSNs: 1369-5274

Abstract

Abstract : The discovery and development of microbial natural products (MNPs) have played pivotal roles in the fields of human medicine and its related biotechnology sectors over the past several decades. The post-genomic era has witnessed the development of microbial genome mining approaches to isolate previously unsuspected MNP biosynthetic gene clusters (BGCs) hidden in the genome, followed by various BGC awakening techniques to visualize compound production. Additional microbial genome engineering techniques have allowed higher MNP production titers, which could complement a traditional culture-based MNP chasing approach. Here, we describe recent developments in the MNP research paradigm, including microbial genome mining, NP BGC activation, and NP overproducing cell factory design.