Development, qualification, validation and application of the Ames test using a VITROCELL® VC10® smoke exposure system. (2018)

• Record Type: Journal Article
• Authors: Fowler, Kathy; Fields, Wanda; Hargreaves, Victoria; Reeve, Lesley; Bombick, Betsy
• Is Part Of: Toxicology reports. Volume 5(2018)
• Page Start: 542
• Page End: 551
• Publication Date: 2018
• Subjects: Whole smoke -- Smoke exposure system -- Ames test -- 3R4F -- Eclipse -- Mutagenicity -- Tobacco heating product; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2018.04.003
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Smoke-induced mutagenicity at air agar interface was developed and validated. The VITROCELL ® VC10 ® system was validated by equipment qualification protocols. Differentiation of mutagenicity by 3R4F and Eclipse cigarettes was demonstrated. Abstract: The Ames test has established use in the assessment of potential mutagenicity of tobacco products but has generally been performed using partitioned exposures (e.g. total particulate matter [TPM], gas vapor phase [GVP]) rather than whole smoke (WS). The VITROCELL ® VC10 ® smoke exposure system offers multiple platforms for air liquid interface (ALI), or air agar interface (AAI) in the case of the Ames test exposure to mimic in vivo -like conditions for assessing the toxicological impact of fresh WS in in vitro assays. The goals of this study were to 1) qualify the VITROCELL ® VC10 ® to demonstrate functionality of the system, 2) develop and validate the Ames test following WS exposure with the VITROCELL ® VC10 ® and 3) assess the ability of the Ames test to differentiate between a reference combustible product (3R4F Kentucky reference cigarette) and a primarily tobacco heating product (Eclipse). Based on critical function assessments, the VITROCELL ® VC10 ® was demonstrated to be fit for the purpose of consistent generation of WS. Assay validation was conducted for 5 bacterial strains (TA97, TA98, TA100, TA1535 and TA102) and reproducible exposure–related changes in revertants were observed for TA98 and TA100 in the presence of rat liver S-9 following exposure to 3R4F WS. In the comparative studies, exposure-related changes in in vitro mutagenicity following exposure of TA98 and TA100 in the presence of S9 to both 3R4F and Eclipse WS were observed, with the response for Eclipse being significantly less than that for 3R4F (p < 0.001) which is consistent with the fewer chemical constituents liberated by primarily-heating the product.