Aquimarins, Peptide Antibiotics with Amino‐Modified C‐Termini from a Sponge‐Derived Aquimarina sp. Bacterium. (29th December 2021)

• Record Type: Journal Article
• Authors: Dieterich, Cora L.; Probst, Silke I.; Ueoka, Reiko; Sandu, Ioana; Schäfle, Daniel; Molin, Michael Dal; Minas, Hannah A.; Costa, Rodrigo; Oxenius, Annette; Sander, Peter; Piel, Jörn
• Is Part Of: Angewandte Chemie. Volume 134:Number 8(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-29
• Subjects: Antibiotics -- Bacteria -- Natural products -- Nonribosomal peptides -- Sponges; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202115802
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Genome mining and bioactivity studies suggested the sponge‐derived bacterium Aquimarina sp. Aq135 as a producer of new antibiotics. Activity‐guided isolation identified antibacterial peptides, named aquimarins, featuring a new scaffold with an unusual C‐terminal amino group and chlorine moieties. Responsible for the halogenation is the Fe II /α‐ketoglutarate‐dependent chlorinase AqmA that halogenates up to two isoleucine residues in a carrier protein‐dependent fashion. Total syntheses of two natural aquimarins and eight non‐natural variants were developed. Structure–activity relationship (SAR) studies with these compounds showed that the synthetically more laborious chlorinations are not required for antibacterial activity but enhance cytotoxicity. In contrast, variants lacking the C‐terminal amine were virtually inactive, suggesting diamines similar to the terminal aquimarin residue as candidate building blocks for new peptidomimetic antibiotics. Abstract : Potion from the ocean: New peptide antibiotics from a sponge‐derived bacterium are reported, featuring chlorination and an unusual amino‐capped C‐terminus. Studies on isolation, structure elucidation, biosynthesis, total synthesis, and structure–activity relationships are presented.