The reliability and validity of triceps surae muscle volume assessment using freehand three‐dimensional ultrasound in typically developing infants. Issue 3 (24th October 2021)

• Record Type: Journal Article
• Authors: Williams, Sîan A.; Bell, Matthew; Kim, Hyun K.; Salim Al Masruri, Ghaliya; Stott, N. Susan; Fernandez, Justin; Mirjalili, S. Ali
• Is Part Of: Journal of anatomy. Volume 240:Issue 3(2022)
• Page Start: 567
• Page End: 578
• Publication Date: 2021-10-24
• Subjects: child -- muscle volume -- reliability -- statistical shape modelling -- ultrasound -- validity; Anatomy -- Periodicals; 571.3
• DOI: 10.1111/joa.13565
• Languages: English
• ISSNs: 0021-8782

Abstract

Abstract: This study assessed the intra‐acquirer, intra‐ and inter‐processor reliability, and validity of the in vivo assessment of the medial gastrocnemius (MG), lateral gastrocnemius (LG) and soleus (SOL) muscle volumes using freehand 3D ultrasound (3DUS) in typically developing infants. Reliability assessments of freehand 3DUS were undertaken in infants across three ages groups: three, six and twelve months of age, with validity testing completed against magnetic resonance imaging (MRI) in infants at 3 months of age. Freehand 3DUS scanning was carried out by a single acquirer, with two independent processors manually segmenting images to render volumes. MRI images were segmented independently by a separate processor, with the volumes compared to those obtained via freehand 3DUS. Reliability was assessed using intraclass correlation (ICC), coefficient of variance (CV) and minimal detectable change (MDC) across each assessment time point. Validity was assessed using the limits of agreement. ICCs for intra‐acquirer reliability of the acquisition process for freehand 3DUS ranged from 0.91 to 0.99 across all muscles. ICCs for intra‐processor and inter‐processor reliability for the segmentation process of freehand 3DUS ranged from 0.80 to 0.98 across all muscles. Acceptable levels of agreement between muscle volume obtained by freehand 3DUS and MRI were found for all muscles; however, freehand 3DUS overestimated muscle volume of MG and LG and underestimate the SOL compared with MRI, with average absolute differences of MG = 0.3 ml, LG = 0.3 ml and Sol = 1.2 ml. Freehand 3DUS is a reliable method for measuring in vivo triceps surae muscle volume in typically developing infants. We conclude that freehand 3DUS is a useful tool to assess changes in muscle volume in response to growth and interventions in infants. Abstract : This study established the intra‐acquirer and inter and intra‐processor reliability of freehand three‐dimensional (3D) ultrasound to measure in vivo muscle volume in infants 3–12 months of age, as well as the validity (compared with MRI) in 3‐month‐old infants. The spatial error map developed from our statistical shape analysis showed the sources of segmentation error for both the Lateral and Medial Gastrocnemius, and Soleus muscle between two acquirers.