Developmental potential of cryopreserved gonadal germ cells from 7-day-old chick embryos recovered using the PBS(-) method. Issue 1 (2nd January 2022)

• Record Type: Journal Article
• Authors: Nakajima, Y.; Asano, A.; Tajima, A.
• Is Part Of: British poultry science. Volume 63:Issue 1(2022)
• Page Start: 46
• Page End: 53
• Publication Date: 2022-01-02
• Subjects: Cryopreservation -- germline chimera -- gonadal germ cells (GGCs) -- spermatozoa -- 7-day-old chick embryo -- reproduction -- endangered species -- genetic conservation programme; Poultry -- Periodicals; Poultry industry -- Periodicals; 636.500941
• DOI: 10.1080/00071668.2021.1960952
• Languages: English
• ISSNs: 0007-1668

Abstract

ABSTRACT: 1. A series of experiments were conducted to examine the developmental potential of cryopreserved gonadal germ cells (GGCs) recovered from both males and females on embryo day 7 (7 d-GGCs) using the PBS(-) method. Germline chimeras were produced by transferring 200 frozen/unfrozen 7 d-GGCs recovered from female/male Rhode Island Red (RIR) embryos into the dorsal aorta of 2-day-old female and male white leghorn (WL) embryos. 2. Germ-cell recipient embryos were hatched and raised to sexual maturity and progeny testing was conducted by mating with RIR of the opposite sex. Brown-feathered progeny chicks were hatched in all eight possible progeny testing combinations, except for male GGC recipients produced by transferring female GGCs. Furthermore, brown-feathered progeny chicks were hatched when frozen-thawed sperm from male germline chimeras, produced by transferring unfrozen 7d-GGCs, were inseminated in normal female RIR and female WL germline chimeras. 3. The results indicated that cryopreserved female/male GGCs from 7-day-old chick embryos, recovered using the PBS(-) method, were fully capable of developing into normal spermatozoa and ova in the gonad of recipient embryos under appropriate GGC donor/recipient combinations.