Development of a specific monoclonal antibody-based icELISA for detection of arecoline in traditional Chinese medicines and fresh areca nuts. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Wang, Yunhe; Ding, Mengying; Ma, Huaqing; Wu, Jiao; Zhao, Hongwei; Wan, Yinglang
• Is Part Of: Food and agricultural immunology. Volume 33:Issue 1(2022)
• Page Start: 113
• Page End: 126
• Publication Date: 2022-12-31
• Subjects: Arocoline -- caidine -- monoclonal antibody -- icELISA -- immunoassay; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2021.2025347
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Arecoline, the dominant alkaloid existing in areca nuts, is an addictive substance and classified as a Group 2B potential human carcinogen. Currently, the detection of arecoline is mostly dependent on chromatography-based approaches, which are time-consuming and expensive. We used arecaidine as a hapten to produce a highly specific monoclonal antibody (mAb) against arecoline. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed using the mAb-A5H12, to detect arecoline in traditional Chinese medicines and fresh areca nuts. The icELISA indicated that the half maximum inhibition concentration (IC50) for arecoline was 67.9 ng/mL, with a working range of 10.1–502.6 ng/mL and a limit of detection (LOD) of 3.6 ng/mL. High-performance liquid chromatographic (HPLC) confirmed the accuracy and the working range of icELISA, suggesting that the icELISA approach based on the arecoline specific antibody could be a widely applicable and easy operation method in detection of arecoline in foods and medicines.